| Title: | Metabolomic profiling of plant tissues |
| Author(s): | Rambla JL; Lopez-Gresa MP; Belles JM; Granell A; |
| Address: | "Instituto de Biologia Molecular y Celular de Plantas, CSIC - Universidad Politecnica de Valencia, Ingeniero Fausto Elio s/n., 46022, Valencia, Spain" |
| DOI: | 10.1007/978-1-4939-2444-8_11 |
| ISSN/ISBN: | 1940-6029 (Electronic) 1064-3745 (Linking) |
| Abstract: | "Metabolomics is a powerful discipline aimed at a comprehensive and global analysis of the metabolites present in a cell, tissue, or organism, and to which increasing attention has been paid in the last few years. Given the high diversity in physical and chemical properties of plant metabolites, not a single method is able to analyze them all.Here we describe two techniques for the profiling of two quite different groups of metabolites: polar and semi-polar secondary metabolites, including many of those involved in plant response to biotic and abiotic stress, and volatile compounds, which include those responsible of most of our perception of food flavor. According to these techniques, polar and semi-polar metabolites are extracted in methanol, separated by liquid chromatography (UPLC), and detected by a UV-VIS detector (PDA) and a time-of-flight (ToF) mass spectrometer. Volatile compounds, on the other hand, are extracted by headspace solid phase microextraction (HS-SPME), and separated and detected by gas chromatography coupled to mass spectrometry (GC-MS)" |
| Keywords: | "Gas Chromatography-Mass Spectrometry *Metabolome *Metabolomics/methods Plants/*metabolism Solid Phase Microextraction/methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods Volatile Organic Compounds;" |
| Notes: | "MedlineRambla, Jose L Lopez-Gresa, M P Belles, J M Granell, Antonio eng 2015/03/12 Methods Mol Biol. 2015; 1284:221-35. doi: 10.1007/978-1-4939-2444-8_11" |
