| Title: | Fecal microbiome and volatile organic compound metabolome in obese humans with nonalcoholic fatty liver disease |
| Author(s): | Raman M; Ahmed I; Gillevet PM; Probert CS; Ratcliffe NM; Smith S; Greenwood R; Sikaroodi M; Lam V; Crotty P; Bailey J; Myers RP; Rioux KP; |
| Address: | "Department of Medicine, Division of Gastroenterology and Hepatology, University of Calgary, Calgary, Alberta, Canada. mkothand@ucalgary.ca" |
| Journal Title: | Clin Gastroenterol Hepatol |
| DOI: | 10.1016/j.cgh.2013.02.015 |
| ISSN/ISBN: | 1542-7714 (Electronic) 1542-3565 (Linking) |
| Abstract: | "BACKGROUND & AIMS: The histopathology of nonalcoholic fatty liver disease (NAFLD) is similar to that of alcoholic liver disease. Colonic bacteria are a source of many metabolic products, including ethanol and other volatile organic compounds (VOC) that may have toxic effects on the human host after intestinal absorption and delivery to the liver via the portal vein. Recent data suggest that the composition of the gut microbiota in obese human beings is different from that of healthy-weight individuals. The aim of this study was to compare the colonic microbiome and VOC metabolome of obese NAFLD patients (n = 30) with healthy controls (n = 30). METHODS: Multitag pyrosequencing was used to characterize the fecal microbiota. Fecal VOC profiles were measured by gas chromatography-mass spectrometry. RESULTS: There were statistically significant differences in liver biochemistry and metabolic parameters in NAFLD. Deep sequencing of the fecal microbiome revealed over-representation of Lactobacillus species and selected members of phylum Firmicutes (Lachnospiraceae; genera, Dorea, Robinsoniella, and Roseburia) in NAFLD patients, which was statistically significant. One member of phylum Firmicutes was under-represented significantly in the fecal microbiome of NAFLD patients (Ruminococcaceae; genus, Oscillibacter). Fecal VOC profiles of the 2 patient groups were different, with a significant increase in fecal ester compounds observed in NAFLD patients. CONCLUSIONS: A significant increase in fecal ester VOC is associated with compositional shifts in the microbiome of obese NAFLD patients. These novel bacterial metabolomic and metagenomic factors are implicated in the etiology and complications of obesity" |
| Keywords: | Adult Biota Case-Control Studies Fatty Liver/complications/*pathology Feces/*chemistry/*microbiology Female Gas Chromatography-Mass Spectrometry Humans Male *Metabolome *Microbiota Middle Aged Non-alcoholic Fatty Liver Disease Obesity/complications/*patho; |
| Notes: | "MedlineRaman, Maitreyi Ahmed, Iftikhar Gillevet, Patrick M Probert, Chris S Ratcliffe, Norman M Smith, Steve Greenwood, Rosemary Sikaroodi, Masoumeh Lam, Victor Crotty, Pam Bailey, Jennifer Myers, Robert P Rioux, Kevin P eng Canadian Institutes of Health Research/Canada Research Support, Non-U.S. Gov't 2013/03/05 Clin Gastroenterol Hepatol. 2013 Jul; 11(7):868-75.e1-3. doi: 10.1016/j.cgh.2013.02.015. Epub 2013 Feb 27" |
