| Title: | "The isolation of biologically active mating pheromone, a-factor, from the yeast, Saccharomyces cerevisiae" |
| Author(s): | Proteau G; Gelaznikas R; Merante F; |
| Address: | "Department of Zoology, Erindale College, University of Toronto, Mississauga, Ontario, Canada" |
| Journal Title: | Biochem Biophys Res Commun |
| DOI: | 10.1016/0006-291x(90)91257-s |
| ISSN/ISBN: | 0006-291X (Print) 0006-291X (Linking) |
| Abstract: | "Haploid cell-types of baker's yeast, Saccharomyces cerevisiae, secrete pheromones which are essential for conjugation. Recently a putative structure for the elusive a-factor pheromone has been reported. In this report we present a procedure to obtain a-factor from batch cultures of cells using hydrophobic Amberlite XAD-2 resin in the growth medium with subsequent differential washings of the resin with organic solvents. We have determined the biological activity of the a-factor preparation by verifying that there is an increase in transcription of the a-factor receptor gene, STE3, by Northern analysis of STE3 mRNA before and after exposure of the appropriate cell type to a-factor. Furthermore, a beta-galactosidase assay of the putative receptor gene fused to the lacZ gene, coding for beta-galactosidase (STE3-lacZ), was done to quantify the biological activity of the a-factor" |
| Keywords: | "Amino Acid Sequence Ion Exchange Resins Mating Factor Molecular Sequence Data Peptides/analysis/*isolation & purification/metabolism Pheromones/analysis/*isolation & purification/metabolism Polystyrenes Receptors, Cell Surface/biosynthesis/metabolism Rece;" |
| Notes: | "MedlineProteau, G Gelaznikas, R Merante, F eng Research Support, Non-U.S. Gov't 1990/07/16 Biochem Biophys Res Commun. 1990 Jul 16; 170(1):182-6. doi: 10.1016/0006-291x(90)91257-s" |
