« Previous AbstractNew approaches: pheromones and related chemicals in some USDA insect control programs    Next AbstractMultiple stir bar sorptive extraction combined with gas chromatography-mass spectrometry analysis for a tentative identification of bacterial volatile and/or semi-volatile metabolites »

Molecules

Title:		Comparison of Stir Bar Sorptive Extraction and Solid Phase Microextraction of Volatile and Semi-Volatile Metabolite Profile of Staphylococcus Aureus
Author(s):		Berrou K; Dunyach-Remy C; Lavigne JP; Roig B; Cadiere A;
Address:		"EA7352 CHROME, University of Nimes, Rue du Dr G. Salan, 30021 Nimes CEDEX 1, France. Institut National de la Sante et de la Recherche Medicale, U1047, Universite Montpellier, UFR de Medecine, 30908 Nimes, France. Department of Microbiology, CHU Nimes, Universite Montpellier, 30029 Nimes, France"
Journal Title:		Molecules
Year:		2019
Volume:		20191223
Issue:		1
Page Number:		-
DOI:		10.3390/molecules25010055
ISSN/ISBN:		1420-3049 (Electronic) 1420-3049 (Linking)
Abstract:		"For the analysis of volatile bacterial compounds, solid phase microextraction (SPME) is currently the most widely used metabolite concentration technique. Recently, the potential of stir bar sorptive extraction (SBSE) for this use has been demonstrated. These two approaches were therefore used in combination with gas-chromatography coupled with mass-spectrometry (GC-MS) for the analysis of volatile and semi-volatile bacterial compounds produced by Staphylococcus aureus. In both cases, SPME and SBSE/headspace sorptive extraction (HSSE) enrichment was carried out in two coating phases. A whole analytical and statistical process was developed to differentiate the metabolites produced from the metabolites consumed. The results obtained with SBSE/HSSE and SPME were compared and showed the recovery of 90% of the compounds by SBSE/HSSE. In addition, we were able to detect the production of 12 volatile/semi-volatile compounds by S. aureus, six of which had never been reported before. The extraction by SBSE/HSSE showed higher concentration capacities and greater sensitivity than SPME concerning bacterial compounds, suggesting that this technique may therefore become the new preferred option for bacterial volatile and semi-volatile compound analysis"
Keywords:		"Amines/chemistry/isolation & purification Gas Chromatography-Mass Spectrometry Hydrocarbons, Aromatic/*chemistry/isolation & purification Metabolome/genetics Solid Phase Microextraction Staphylococcal Infections/metabolism/*microbiology Staphylococcus aur;"
Notes:		"MedlineBerrou, Kevin Dunyach-Remy, Catherine Lavigne, Jean-Philippe Roig, Benoit Cadiere, Axelle eng Switzerland 2019/12/28 Molecules. 2019 Dec 23; 25(1):55. doi: 10.3390/molecules25010055"