| Title: | "Attraction, mobility, and preference by Lasioderma serricorne (Coleoptera: Ptinidae) to microbially-mediated volatile emissions by two species of fungi in stored grain" |
| Author(s): | Ponce MA; Sierra P; Maille JM; Kim TN; Scully ED; Morrison WR; |
| Address: | "Department of Entomology, Kansas State University, 123 W. Waters Hall, 1603 Old Claflin Place, Manhattan, KS, 66506, USA. marco26@ksu.edu. Biology Department, Kalamazoo College, 1200 Academy St., Kalamazoo, MI, 49006, USA. Department of Entomology, Kansas State University, 123 W. Waters Hall, 1603 Old Claflin Place, Manhattan, KS, 66506, USA. USDA, Agricultural Research Service, Center for Grain and Animal Health Research, 1515 College Ave., Manhattan, KS, 66502, USA" |
| DOI: | 10.1038/s41598-023-32973-y |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Insects and microbes are known to interact in a variety of ways at food facilities, compounding damage. However, little research has explicated how specific common fungal species affect the behavior of the cosmopolitan secondary stored product pest, Lasioderma serricorne. Enhanced knowledge about attraction to microbially-produced volatile organic compounds (MVOCs) may be used to manipulate insect behavior. Aspergillus flavus and Fusarium verticillioides are two common, widespread pre- and postharvest fungi on small cereals that produce aflatoxins and fumonisins, respectively, while directly competing with each other for nutrients. Our goals were to (1) characterize the volatile emissions from grain inoculated by A. flavus or F. verticillioides derived from the cuticle of L. serricorne compared to uninoculated and sanitized grain, and (2) understand how MVOCs from each fungal species affects mobility, attraction, and preference by L. serricorne. Headspace collection revealed that the F. verticillioides- and A. flavus-inoculated grain produced significantly different volatiles compared to sanitized grain or the positive control. Changes in MVOC emissions affected close-range foraging during an Ethovision movement assay, with a greater frequency of entering and spending time in a small zone with kernels inoculated with A. flavus compared to other treatments. In the release-recapture assay, MVOCs were found to be attractive to L. serricorne at longer distances in commercial pitfall traps. There was no preference shown among semiochemical stimuli in a still-air, four-way olfactometer. Overall, our study suggests that MVOCs are important for close- and long-range orientation of L. serricorne during foraging, and that MVOCs may have the potential for inclusion in behaviorally-based tactics for this species" |
| Keywords: | Animals *Coleoptera Edible Grain Insecta Pheromones Fungi; |
| Notes: | "MedlinePonce, Marco A Sierra, Petra Maille, Jacqueline M Kim, Tania N Scully, Erin D Morrison, William R 3rd eng Research Support, U.S. Gov't, Non-P.H.S. England 2023/04/16 Sci Rep. 2023 Apr 15; 13(1):6176. doi: 10.1038/s41598-023-32973-y" |
