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Toxicol In Vitro

Title:		Cytotoxic mechanism of Baccharis milleflora (Less.) DC. essential oil
Author(s):		Pereira CB; Kanunfre CC; Farago PV; Borsato DM; Budel JM; de Noronha Sales Maia BHL; Campesatto EA; Sartoratto A; Miguel MD; Miguel OG;
Address:		"Postgraduate Program in Pharmaceutical Sciences, Federal University of Parana, 632 Prefeito Lothario Meissner Avenue, Zip Code 80210-170 Curitiba, PR, Brazil. Postgraduate Program in Biomedical Sciences, State University of Ponta Grossa, 4748 Carlos Cavalcanti Avenue, Zip Code 84030-900 Ponta Grossa, PR, Brazil. Postgraduate Program in Pharmaceutical Sciences, State University of Ponta Grossa, 4748 Carlos Cavalcanti Avenue, Zip Code 84030-900 Ponta Grossa, PR, Brazil. Electronic address: pvfarago@uepg.br. Postgraduate Program in Pharmaceutical Sciences, State University of Ponta Grossa, 4748 Carlos Cavalcanti Avenue, Zip Code 84030-900 Ponta Grossa, PR, Brazil. Postgraduate Program in Chemistry, Federal University of Parana, Polytechnic Center, P.O. Box 19081, Zip Code 81531-990 Curitiba, PR, Brazil. Laboratory of Pharmacology and Immunity, Institute of Biological and Health Sciences, Federal University of Alagoas, Lourival Melo Mota Avenue, Zip Code 57072-900 Maceio, AL, Brazil. Organic Chemistry and Pharmaceutical Division, Research Center for Chemistry, Biology and Agriculture, University of Campinas, P.O. Box 6171, Zip Code 13083-970 Campinas, SP, Brazil"
Journal Title:		Toxicol In Vitro
Year:		2017
Volume:		20170502
Issue:
Page Number:		214 - 221
DOI:		10.1016/j.tiv.2017.04.031
ISSN/ISBN:		1879-3177 (Electronic) 0887-2333 (Linking)
Abstract:		"The aim of this study was to investigate the cytotoxic activity of the essential oil from cladodes of Bacharis milleflora in relation to Jurkat, Raji and HL-60 cells, as well as exploring the cell mechanisms in order to elucidate how the cytotoxic process occurs. The presence of the following volatile compounds was detected by GC-MS: bicyclogermacrene (12.16%), germacrene D (11.18%), (E)-caryophyllene (9.28%), and alpha-humulene (8.05%). In general, IC(50) values lower than 50mug/mL were obtained for all the tumor cells at 24, 48 and 72h by MTT assay. The decrease in cell DNA content was demonstrated due to the inhibition of the proliferation of Jurkat, Raji and HL-60 cells by B. milleflora essential oil. In particular, Raji cells presented the greatest inhibition of cell proliferation and they were subsequently used to investigate cell death mechanisms. B. milleflora essential oil promoted G0/G1 arrest and also induced cell fragmentation, which was represented by an increase in the sub-G0 population, indicating cell death induced by apoptosis. The selectivity index was 3.97. Necrotic cell death, coupled with low levels of apoptotic cell death, was observed by conventional EB/AO and Hoechst 33342 staining assays, demonstrating that this essential oil acts via both necrotic and apoptotic mechanisms"
Keywords:		"Apoptosis/drug effects *Baccharis Cell Line Cell Proliferation/drug effects Cell Survival/drug effects Cells, Cultured DNA/metabolism Humans Lymphocytes/drug effects Male Necrosis/chemically induced Oils, Volatile/chemistry/*toxicity Phytochemicals/analys;"
Notes:		"MedlinePereira, Camila Bugnotto Kanunfre, Carla Cristine Farago, Paulo Vitor Borsato, Debora Maria Budel, Jane Manfron de Noronha Sales Maia, Beatriz Helena Lameiro Campesatto, Eliane Aparecida Sartoratto, Adilson Miguel, Marilis Dallarmi Miguel, Obdulio Gomes eng England 2017/05/10 Toxicol In Vitro. 2017 Aug; 42:214-221. doi: 10.1016/j.tiv.2017.04.031. Epub 2017 May 2"