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Plant Physiol Biochem
| Title: | "Cytokinins act synergistically with heat acclimation to enhance rice thermotolerance affecting hormonal dynamics, gene expression and volatile emission" |
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| Author(s): | Prerostova S; Rezek J; Jarosova J; Lacek J; Dobrev P; Marsik P; Gaudinova A; Knirsch V; Dolezal K; Plihalova L; Vanek T; Kieber J; Vankova R; |
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| Address: | "Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany, Czech Academy of Sciences, Rozvojova 263, 165 02, Prague, Czech Republic. Electronic address: prerostova@ueb.cas.cz. Laboratory of Plant Biotechnologies, Institute of Experimental Botany, Czech Academy of Sciences, Rozvojova 313, 165 02, Prague, Czech Republic. Electronic address: rezek@ueb.cas.cz. Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany, Czech Academy of Sciences, Rozvojova 263, 165 02, Prague, Czech Republic. Electronic address: jarosova@ueb.cas.cz. Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany, Czech Academy of Sciences, Rozvojova 263, 165 02, Prague, Czech Republic. Electronic address: lacek@ueb.cas.cz. Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany, Czech Academy of Sciences, Rozvojova 263, 165 02, Prague, Czech Republic. Electronic address: dobrev@ueb.cas.cz. Laboratory of Plant Biotechnologies, Institute of Experimental Botany, Czech Academy of Sciences, Rozvojova 313, 165 02, Prague, Czech Republic. Electronic address: marsik@ueb.cas.cz. Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany, Czech Academy of Sciences, Rozvojova 263, 165 02, Prague, Czech Republic. Electronic address: gaudinova@ueb.cas.cz. Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany, Czech Academy of Sciences, Rozvojova 263, 165 02, Prague, Czech Republic. Electronic address: knirsch@ueb.cas.cz. Laboratory of Growth Regulators, Institute of Experimental Botany, Czech Academy of Sciences, Slechtitelu 27, 783 71, Olomouc, Czech Republic; Department of Chemical Biology, Faculty of Science, Palacky University, 17. listopadu 1192/12, 779 00, Olomouc, Czech Republic. Electronic address: karel.dolezal@upol.cz. Laboratory of Growth Regulators, Institute of Experimental Botany, Czech Academy of Sciences, Slechtitelu 27, 783 71, Olomouc, Czech Republic; Department of Chemical Biology, Faculty of Science, Palacky University, 17. listopadu 1192/12, 779 00, Olomouc, Czech Republic. Electronic address: lucie.plihalova@upol.cz. Laboratory of Plant Biotechnologies, Institute of Experimental Botany, Czech Academy of Sciences, Rozvojova 313, 165 02, Prague, Czech Republic. Electronic address: vanek@ueb.cas.cz. Department of Biology, University of North Carolina, Chapel Hill, NC, 27599, USA. Electronic address: jkieber@bio.unc.edu. Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany, Czech Academy of Sciences, Rozvojova 263, 165 02, Prague, Czech Republic. Electronic address: vankova@ueb.cas.cz" |
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| Journal Title: | Plant Physiol Biochem |
| Year: | 2023 |
| Volume: | 20230406 |
| Issue: | |
| Page Number: | 107683 - |
| DOI: | 10.1016/j.plaphy.2023.107683 |
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| ISSN/ISBN: | 1873-2690 (Electronic) 0981-9428 (Linking) |
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| Abstract: | "Heat stress is a frequent environmental constraint. Phytohormones can significantly affect plant thermotolerance. This study compares the effects of exogenous cytokinin meta-topolin-9-(tetrahydropyran-2-yl)purine (mT9THP) on rice (Oryza sativa) under control conditions, after acclimation by moderate temperature (A; 37 degrees C, 2h), heat stress (HS; 45 degrees C, 6h) and their combination (AHS). mT9THP is a stable cytokinin derivative that releases active meta-topolin gradually, preventing the rapid deactivation reported after exogenous cytokinin application. Under control conditions, mT9THP negatively affected jasmonic acid in leaves and abscisic and salicylic acids in crowns (meristematic tissue crucial for tillering). Exogenous cytokinin stimulated the emission of volatile organic compounds (VOC), especially 2,3-butanediol. Acclimation upregulated trans-zeatin, expression of stress- and hormone-related genes, and VOC emission. The combination of acclimation and mT9THP promoted the expression of stress markers and antioxidant enzymes and moderately increased VOC emission, including 2-ethylhexyl salicylate or furanones. AHS and HS responses shared some common features, namely, increase of ethylene precursor aminocyclopropane-1-carboxylic acid (ACC), cis-zeatin and cytokinin methylthio derivatives, as well as the expression of heat shock proteins, alternative oxidases, and superoxide dismutases. AHS specifically induced jasmonic acid and auxin indole-3-acetic acid levels, diacylglycerolipids with fewer double bonds, and VOC emissions [e.g., acetamide, lipoxygenase (LOX)-derived volatiles]. Under direct HS, exogenous cytokinin mimicked some positive acclimation effects. The combination of mT9THP and AHS had the strongest thermo-protective effect, including a strong stimulation of VOC emissions (including LOX-derived ones). These results demonstrate for the first time the crucial contribution of volatiles to the beneficial effects of cytokinin and AHS on rice thermotolerance" |
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| Keywords: | Cytokinins/metabolism *Oryza/metabolism Zeatin/metabolism *Thermotolerance *Volatile Organic Compounds Acclimatization Gene Expression Acclimation Cytokinin Heat stress Phytohormone Priming Thermotolerance Volatile organic compound; |
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| Notes: | "MedlinePrerostova, Sylva Rezek, Jan Jarosova, Jana Lacek, Jozef Dobrev, Petre Marsik, Petr Gaudinova, Alena Knirsch, Vojtech Dolezal, Karel Plihalova, Lucie Vanek, Tomas Kieber, Joseph Vankova, Radomira eng France 2023/04/17 Plant Physiol Biochem. 2023 May; 198:107683. doi: 10.1016/j.plaphy.2023.107683. Epub 2023 Apr 6" |
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