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J Insect Physiol

Title:		Identification of olfactory receptor neurons in Uraba lugens (Lepidoptera: Nolidae) and its implications for host range
Author(s):		Park KC; Withers TM; Suckling DM; Better Border Biosecurity C;
Address:		"The New Zealand Institute for Plant & Food Research, PB 4704, Christchurch 8140, New Zealand. Electronic address: kpark@plantandfood.co.nz. Scion, PB 3020, Rotorua, New Zealand. Electronic address: toni.withers@scionresearch.com. The New Zealand Institute for Plant & Food Research, PB 4704, Christchurch 8140, New Zealand. Electronic address: max.suckling@plantandfood.co.nz"
Journal Title:		J Insect Physiol
Year:		2015
Volume:		20150501
Issue:
Page Number:		33 - 46
DOI:		10.1016/j.jinsphys.2015.04.010
ISSN/ISBN:		1879-1611 (Electronic) 0022-1910 (Linking)
Abstract:		"Phytophagous insects detect volatile compounds produced by host and non-host plants, using species-specific sets of olfactory receptor neurons (ORNs). To investigate the relationship between the range of host plants and the profile of ORNs, single sensillum recordings were carried out to identify ORNs and corresponding active compounds in female Uraba lugens (Lepidoptera: Nolidae), an oligophagous eucalypt feeder. Based on the response profiles to 39 plant volatile compounds, 13 classes of sensilla containing 40 classes of ORNs were identified in female U. lugens. More than 95% (163 out of 171) of these sensilla contained 16 classes of ORNs with narrow response spectra, and 62.6% (107 out of 171) 18 classes of ORNs with broad response spectra. Among the specialized ORNs, seven classes of ORNs exhibited high specificity to 1,8-cineole, (+/-)-citronellal, myrcene, (+/-)-linalool and (E)-beta-caryophyllene, major volatiles produced by eucalypts, while nine other classes of ORNs showed highly specialized responses to green leaf volatiles, germacrene D, (E)-beta-farnesene and geranyl acetate that are not produced by most eucalypts. We hypothesize that female U. lugens can recognize their host plants by detecting key host volatile compounds, using a set of ORNs tuned to host volatiles, and discriminate them from non-host plants using another set of ORNs specialized for non-host volatiles. The ORNs with broad response spectra may enhance the discrimination between host and non-host plants by adding moderately selective sensitivity. Based on our finding, it is suggested that phytophagous insects use the combinational input from both host-specific and non-host specific ORNs for locating their host plants, and the electrophysiological characterization of ORN profiles would be useful in predicting the range of host plants in phytophagous insects"
Keywords:		Animals Female Herbivory Host Specificity Moths/*physiology Olfactory Receptor Neurons/*physiology Plants/chemistry Sensilla/physiology Volatile Organic Compounds/*pharmacology Electrophysiology Host plant Host range Olfactory receptor neuron Phytophagous;
Notes:		"MedlinePark, Kye Chung Withers, Toni M Suckling, David Maxwell (B3) eng Research Support, Non-U.S. Gov't England 2015/05/06 J Insect Physiol. 2015 Jul; 78:33-46. doi: 10.1016/j.jinsphys.2015.04.010. Epub 2015 May 1"