| Title: | Helicoverpa zea-Associated Gut Bacteria as Drivers in Shaping Plant Anti-herbivore Defense in Tomato |
| Author(s): | Pan Q; Shikano I; Liu TX; Felton GW; |
| Address: | "Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, China. qjpan@yzu.edu.cn. Department of Entomology and Center for Chemical Ecology, Pennsylvania State University, University Park, Pennsylvania, PA, 16802, USA. qjpan@yzu.edu.cn. Department of Plant and Environmental Protection Sciences, University of Hawai'i at Manoa, Honolulu, HI, 96822, USA. Institute of Entomology, Guizhou University, Guiyang, Guizhou, China. tx.liu@gzu.edu.cn. Department of Entomology and Center for Chemical Ecology, Pennsylvania State University, University Park, Pennsylvania, PA, 16802, USA" |
| DOI: | 10.1007/s00248-023-02232-8 |
| ISSN/ISBN: | 1432-184X (Electronic) 0095-3628 (Linking) |
| Abstract: | "Insect-associated bacteria can mediate the intersection of insect and plant immunity. In this study, we aimed to evaluate the effects of single isolates or communities of gut-associated bacteria of Helicoverpa zea larvae on herbivore-induced defenses in tomato. We first identified bacterial isolates from the regurgitant of field-collected H. zea larvae by using a culture-dependent method and 16S rRNA gene sequencing. We identified 11 isolates belonging to the families Enterobacteriaceae, Streptococcaceae, Yersiniaceae, Erwiniaceae, and unclassified Enterobacterales. Seven different bacterial isolates, namely Enterobacteriaceae-1, Lactococcus sp., Klebsiella sp. 1, Klebsiella sp. 3, Enterobacterales, Enterobacteriaceae-2, and Pantoea sp., were selected based on their phylogenetic relationships to test their impacts on insect-induced plant defenses. We found that the laboratory population of H. zea larvae inoculated with individual isolates did not induce plant anti-herbivore defenses, whereas larvae inoculated with a bacterial community (combination of the 7 bacterial isolates) triggered increased polyphenol oxidase (PPO) activity in tomato, leading to retarded larval development. Additionally, field-collected H. zea larvae with an unaltered bacterial community in their gut stimulated higher plant defenses than the larvae with a reduced gut microbial community. In summary, our findings highlight the importance of the gut microbial community in mediating interactions between herbivores and their host plants" |
| Keywords: | "Humans Animals *Solanum lycopersicum Zea mays Plant Defense Against Herbivory Phylogeny RNA, Ribosomal, 16S/genetics *Moths Larva/microbiology Bacteria/genetics Enterobacteriaceae Herbivory Helicoverpa zea Induced defense Insect-plant interactions Microbi;" |
| Notes: | "MedlinePan, Qinjian Shikano, Ikkei Liu, Tong-Xian Felton, Gary W eng 2023/05/08 Microb Ecol. 2023 Oct; 86(3):2173-2182. doi: 10.1007/s00248-023-02232-8. Epub 2023 May 8" |
