| Title: | Fall armyworm (Spodoptera frugiperda Smith) feeding elicits differential defense responses in upland and lowland switchgrass |
| Author(s): | Palmer NA; Basu S; Heng-Moss T; Bradshaw JD; Sarath G; Louis J; |
| Address: | "Wheat, Sorghum, and Forage Research Unit, USDA-ARS, Lincoln, NE, United States of America. Department of Entomology, University of Nebraska-Lincoln, Lincoln, NE, United States of America. Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, United States of America" |
| DOI: | 10.1371/journal.pone.0218352 |
| ISSN/ISBN: | 1932-6203 (Electronic) 1932-6203 (Linking) |
| Abstract: | "Switchgrass (Panicum virgatum L.) is a low input, high biomass perennial grass being developed for the bioenergy sector. Upland and lowland cultivars can differ in their responses to insect herbivory. Fall armyworm [FAW; Spodoptera frugiperda JE Smith (Lepidoptera: Noctuidae)] is a generalist pest of many plant species and can feed on switchgrass as well. Here, in two different trials, FAW larval mass were significantly reduced when fed on lowland cultivar Kanlow relative to larvae fed on upland cultivar Summer plants after 10 days. Hormone content of plants indicated elevated levels of the plant defense hormone jasmonic acid (JA) and its bioactive conjugate JA-Ile although significant differences were not observed. Conversely, the precursor to JA, 12-oxo-phytodienoic acid (OPDA) levels were significantly different between FAW fed Summer and Kanlow plants raising the possibility of differential signaling by OPDA in the two cultivars. Global transcriptome analysis revealed a stronger response in Kanlow plant relative to Summer plants. Among these changes were a preferential upregulation of several branches of terpenoid and phenylpropanoid biosynthesis in Kanlow plants suggesting that enhanced biosynthesis or accumulation of antifeedants could have negatively impacted FAW larval mass gain on Kanlow plants relative to Summer plants. A comparison of the switchgrass-FAW RNA-Seq dataset to those from maize-FAW and switchgrass-aphid interactions revealed that key components of plant responses to herbivory, including induction of JA biosynthesis, key transcription factors and JA-inducible genes were apparently conserved in switchgrass and maize. In addition, these data affirm earlier studies with FAW and aphids that the cultivar Kanlow can provide useful genetics for the breeding of switchgrass germplasm with improved insect resistance" |
| Keywords: | "Animals Biomass *Feeding Behavior Gene Expression Regulation, Plant Herbivory Larva Panicum/chemistry/*genetics/metabolism Plant Growth Regulators/chemistry/genetics *Spodoptera Transcriptome;" |
| Notes: | "MedlinePalmer, Nathan A Basu, Saumik Heng-Moss, Tiffany Bradshaw, Jeffrey D Sarath, Gautam Louis, Joe eng P20 GM103427/GM/NIGMS NIH HHS/ P20 RR016469/RR/NCRR NIH HHS/ P20 RR018788/RR/NCRR NIH HHS/ P20 GM103471/GM/NIGMS NIH HHS/ S10 RR027754/RR/NCRR NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2019/06/14 PLoS One. 2019 Jun 13; 14(6):e0218352. doi: 10.1371/journal.pone.0218352. eCollection 2019" |
