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Methods Mol Biol


Title:A Method for Obtaining Large Populations of Synchronized Caenorhabditis elegans Dauer Larvae
Author(s):Ow MC; Hall SE;
Address:"Department of Biology, Syracuse University, 107 College Place, Room 110, Syracuse, NY, 13244, USA. Department of Biology, Syracuse University, 107 College Place, Room 110, Syracuse, NY, 13244, USA. shall@syr.edu"
Journal Title:Methods Mol Biol
Year:2015
Volume:1327
Issue:
Page Number:209 - 219
DOI: 10.1007/978-1-4939-2842-2_15
ISSN/ISBN:1940-6029 (Electronic) 1064-3745 (Linking)
Abstract:"The C. elegans dauer is an attractive model with which to investigate fundamental biological questions, such as how environmental cues are sensed and are translated into developmental decisions through a series of signaling cascades that ultimately result in a transformed animal. Here we describe a simple method of using egg white plates to obtain highly synchronized purified dauers that can be used in downstream applications requiring large quantities of dauers or postdauer animals"
Keywords:Animals Caenorhabditis elegans/*physiology Larva Caenorhabditis elegans Dauer Diapause Egg white plate Pheromone Postdauer Stress;
Notes:"MedlineOw, Maria C Hall, Sarah E eng 2015/10/02 Methods Mol Biol. 2015; 1327:209-19. doi: 10.1007/978-1-4939-2842-2_15"

 
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