| Title: | Array-Based Rational Design of Short Peptide Probe-Derived from an Anti-TNT Monoclonal Antibody |
| Author(s): | Okochi M; Muto M; Yanai K; Tanaka M; Onodera T; Wang J; Ueda H; Toko K; |
| Address: | "Department of Chemical Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of Technology , 2-12-1, O-okayama, Meguro-ku, Tokyo 152-8552, Japan. JST, ImPACT, Sanban-cho 5, Chiyoda-ku, Tokyo 102-0075, Japan. Research and Development Center for Taste and Odor Sensing, Kyushu University, Fukuoka , 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan. Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology , R1-18, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8503, Japan. Graduate School of Information Science and Electrical Engineering, Kyushu University , 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan" |
| DOI: | 10.1021/acscombsci.7b00035 |
| ISSN/ISBN: | 2156-8944 (Electronic) 2156-8944 (Linking) |
| Abstract: | "Complementarity-determining regions (CDRs) are sites on the variable chains of antibodies responsible for binding to specific antigens. In this study, a short peptide probe for recognition of 2,4,6-trinitrotoluene (TNT), was identified by testing sequences derived from the CDRs of an anti-TNT monoclonal antibody. The major TNT-binding site in this antibody was identified in the heavy chain CDR3 by antigen docking simulation and confirmed by an immunoassay using a spot-synthesis based peptide array comprising amino acid sequences of six CDRs in the variable region. A peptide derived from heavy chain CDR3 (RGYSSFIYWF) bound to TNT with a dissociation constant of 1.3 muM measured by surface plasmon resonance. Substitution of selected amino acids with basic residues increased TNT binding while substitution with acidic amino acids decreased affinity, an isoleucine to arginine change showed the greatest improvement of 1.8-fold. The ability to create simple peptide binders of volatile organic compounds from sequence information provided by the immune system in the creation of an immune response will be beneficial for sensor developments in the future" |
| Keywords: | "Amino Acid Sequence Antibodies, Monoclonal/*chemistry Binding Sites Complementarity Determining Regions Humans Immunoassay Peptides/*chemistry Protein Binding Sensitivity and Specificity Surface Plasmon Resonance Trinitrotoluene/*chemistry/immunology 2, 4, ;" |
| Notes: | "MedlineOkochi, Mina Muto, Masaki Yanai, Kentaro Tanaka, Masayoshi Onodera, Takeshi Wang, Jin Ueda, Hiroshi Toko, Kiyoshi eng Research Support, Non-U.S. Gov't 2017/08/29 ACS Comb Sci. 2017 Oct 9; 19(10):625-632. doi: 10.1021/acscombsci.7b00035. Epub 2017 Sep 8" |
