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PLoS One

Title:		Extracellular modulation of the silkmoth sex pheromone receptor activity by cyclic nucleotides
Author(s):		Nakagawa T; Touhara K;
Address:		"Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan"
Journal Title:		PLoS One
Year:		2014
Volume:		20130603
Issue:		6
Page Number:		e63774 -
DOI:		10.1371/journal.pone.0063774
ISSN/ISBN:		1932-6203 (Electronic) 1932-6203 (Linking)
Abstract:		"Odorants and pheromones are essential to insects as chemical cues for finding food or an appropriate mating partner. These volatile compounds bind to olfactory receptors (Ors) expressed by olfactory sensory neurons. Each insect Or functions as a ligand-gated ion channel and is a heteromeric complex that comprises one type of canonical Or and a highly conserved Orco subunit. Because there are many Or types, insect Ors can recognize with high specificity a myriad of chemical cues. Cyclic nucleotides can modulate the activity of insect Or-Orco complexes; however, the mechanism of action of these nucleotides is under debate. Here, we show that cyclic nucleotides, including cAMP and cGMP, interact with the silkmoth sex pheromone receptor complex, BmOr-1-BmOrco, from the outside of the cell and that these nucleotides act as antagonists at low concentrations and weak agonists at high concentrations. These cyclic nucleotides do not compete with the sex pheromone, bombykol, for binding to the BmOr-1 subunit. ATP and GTP also weakly inhibited BmOr-1-BmOrco activity, but D-ribose had no effect; these findings indicated that the purine moiety was crucial for the inhibition. Only the bombykol receptors have been so far shown to be subject to modulation by nucleotide-related compounds, indicating that this responsiveness to these compounds is not common for all insect Or-Orco complexes"
Keywords:		"Animals Bombyx/drug effects/*metabolism Extracellular Space/drug effects/metabolism Fatty Alcohols/pharmacology HEK293 Cells Humans Nucleotides, Cyclic/*pharmacology Protein Subunits/metabolism Receptors, Pheromone/antagonists & inhibitors/*metabolism Sex;"
Notes:		"MedlineNakagawa, Tatsuro Touhara, Kazushige eng Research Support, Non-U.S. Gov't 2013/06/12 PLoS One. 2013 Jun 3; 8(6):e63774. doi: 10.1371/journal.pone.0063774. Print 2014"