| Title: | Functional characterization of olfactory receptors in the Oriental fruit fly Bactrocera dorsalis that respond to plant volatiles |
| Author(s): | Miyazaki H; Otake J; Mitsuno H; Ozaki K; Kanzaki R; Chui-Ting Chieng A; Kah-Wei Hee A; Nishida R; Ono H; |
| Address: | "Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan. Research Center for Advanced Science and Technology, University of Tokyo, Tokyo 153-8904, Japan. JT Biohistory Research Hall, Takatsuki Osaka, 569-1125, Japan. Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia. Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan. Electronic address: onoono@kais.kyoto-u.ac.jp" |
| DOI: | 10.1016/j.ibmb.2018.07.002 |
| ISSN/ISBN: | 1879-0240 (Electronic) 0965-1748 (Linking) |
| Abstract: | "The Oriental fruit fly, Bactrocera dorsalis, is a highly destructive pest of various fruits. The reproductive and host-finding behaviors of this species are affected by several plant semiochemicals that are perceived through chemosensory receptors. However, the chemosensory mechanisms by which this perception occurs have not been fully elucidated. We conducted RNA sequencing analysis of the chemosensory organs of B. dorsalis to identify the genes coding for chemosensory receptors. We identified 60 olfactory receptors (ORs), 17 gustatory receptors and 23 ionotropic receptors-including their homologs and variants-from the transcriptome of male antennae and proboscises. We functionally analyzed ten ORs co-expressed with the obligatory co-receptor ORCO in Xenopus oocytes to identify their ligands. We tested 24 compounds including attractants for several Bactrocera species and volatiles from the host fruits of B. dorsalis. We found that BdorOR13a co-expressed with ORCO responded robustly to 1-octen-3-ol. BdorOR82a co-expressed with ORCO responded significantly to geranyl acetate, but responded weakly to farnesenes (a mixture of isomers) and linalyl acetate. These four compounds were subsequently subjected to behavioral bioassays. When each of the aforementioned compound was presented in combination with a sphere model as a visual cue to adult flies, 1-octen-3-ol, geranyl acetate, and farnesenes significantly enhanced landing behavior in mated females, but not in unmated females or males. These results suggest that the ORs characterized in the present study are involved in the perception of plant volatiles that affect host-finding behavior in B. dorsalis" |
| Keywords: | Acetates/chemistry/pharmacology Acyclic Monoterpenes Animals Arthropod Antennae/cytology/drug effects/physiology Biological Assay Female Gene Expression Regulation Insect Proteins/*genetics/metabolism Male Monoterpenes/chemistry/pharmacology Octanols/chem; |
| Notes: | "MedlineMiyazaki, Hitomi Otake, Jun Mitsuno, Hidefumi Ozaki, Katsuhisa Kanzaki, Ryohei Chui-Ting Chieng, Anna Kah-Wei Hee, Alvin Nishida, Ritsuo Ono, Hajime eng Research Support, Non-U.S. Gov't England 2018/07/22 Insect Biochem Mol Biol. 2018 Oct; 101:32-46. doi: 10.1016/j.ibmb.2018.07.002. Epub 2018 Jul 17" |
