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Cell

Title:		"GPA1, a haploid-specific essential gene, encodes a yeast homolog of mammalian G protein which may be involved in mating factor signal transduction"
Author(s):		Miyajima I; Nakafuku M; Nakayama N; Brenner C; Miyajima A; Kaibuchi K; Arai K; Kaziro Y; Matsumoto K;
Address:
Journal Title:		Cell
Year:		1987
Volume:		50
Issue:		7
Page Number:		1011 - 1019
DOI:		10.1016/0092-8674(87)90167-x
ISSN/ISBN:		0092-8674 (Print) 0092-8674 (Linking)
Abstract:		"GPA1 protein of Saccharomyces cerevisiae is homologous to the alpha subunit of mammalian G protein. GPA1 transcript was found in haploid cells but was not detected in diploid cells. Disruption of GPA1 resulted in a haploid-specific lethal phenotype, indicating that GPA1 is a haploid-specific essential gene for cell growth. Upon regulation of expression of GPA1 by the galactose-inducible GAL1 promoter, the loss of GPA1 function was found to lead to cell-cycle arrest at the late G1 phase. Mutants that suppress the lethality of the gpa1::HIS3 mutation showed a sterile phenotype that was not cell-type-specific. These results suggest that GPA1 protein may control the signal for mating-factor-mediated cell-cycle arrest"
Keywords:		"Cell Cycle GTP-Binding Proteins/*genetics/physiology Genes *Genes, Fungal Mating Factor Peptides/physiology Saccharomyces cerevisiae/*genetics/physiology Sequence Homology, Nucleic Acid Species Specificity;"
Notes:		"MedlineMiyajima, I Nakafuku, M Nakayama, N Brenner, C Miyajima, A Kaibuchi, K Arai, K Kaziro, Y Matsumoto, K eng Comparative Study 1987/09/25 Cell. 1987 Sep 25; 50(7):1011-9. doi: 10.1016/0092-8674(87)90167-x"