| Title: | Genetic similarity among pheromone and voltinism races of Ostrinia nubilalis (Hubner) (Lepidoptera: Crambidae) |
| Author(s): | Marcon PC; Taylor DB; Mason CE; Hellmich RL; Siegfried BD; |
| Address: | "Department of Entomology, University of Nebraska-Lincoln, 68583-0816, USA" |
| DOI: | 10.1046/j.1365-2583.1999.820213.x |
| ISSN/ISBN: | 0962-1075 (Print) 0962-1075 (Linking) |
| Abstract: | "The genetic variability of seven European corn borer populations, Ostrinia nubilalis, from North America and Europe was assessed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis and DNA sequencing. The nuclear ribosomal internal transcribed spacer 1 (ITS-1) region (approximately 500 base pair [bp]) and four mitochondrial (mtDNA) regions (1550 bp total) were examined. The smartweed borer, Ostrinia obumbratalis, and south-Western corn borer, Diatraea grandiosella, were used for comparisons. Of 106 restriction sites identified (80 in mtDNA and 26 in ITS-1), none differentiated geographical populations, pheromone races, or voltine ecotypes of the European corn borer. The lack of variation in the ITS-1 of European corn borer was confirmed by DNA sequence analysis. The genetic similarity of European corn borer populations, despite their wide geographical range and physiological differences, may be explained by a relatively recent origin for the voltinism and pheromone races, gene flow among races, and/or expansion from genetic bottlenecks" |
| Keywords: | "Animals Base Sequence DNA, Complementary *DNA, Mitochondrial Female Insect Proteins/*genetics Molecular Sequence Data Moths/*genetics Pheromones/*genetics Polymorphism, Restriction Fragment Length Protein Isoforms Sequence Analysis, DNA;" |
| Notes: | "MedlineMarcon, P C Taylor, D B Mason, C E Hellmich, R L Siegfried, B D eng Research Support, Non-U.S. Gov't England 1999/06/24 Insect Mol Biol. 1999 May; 8(2):213-21. doi: 10.1046/j.1365-2583.1999.820213.x" |
