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J Sep Sci

Title:		Comprehensive two-dimensional gas chromatography coupled with static headspace sampling to analyze volatile compounds: application to almonds
Author(s):		Manzano P; Diego JC; Bernal JL; Nozal MJ; Bernal J;
Address:		"I.U. CINQUIMA, Analytical Chemistry Group, University of Valladolid, Valladolid, Spain"
Journal Title:		J Sep Sci
Year:		2014
Volume:		20140208
Issue:		6
Page Number:		675 - 683
DOI:		10.1002/jssc.201301278
ISSN/ISBN:		1615-9314 (Electronic) 1615-9306 (Linking)
Abstract:		"The hyphenation of static headspace sampling with comprehensive 2D GC equipped with a modulator based on capillary flow technology and a flame ionization detector was used to separate and identify 43 representative target volatile compounds (light hydrocarbons, carbonyls, pyrazines, alcohols, furans, and benzenes) frequently detected in the roasting process of nuts. Five column combinations with differing degrees of orthogonality (one conventional and four inverted phase sets) were tested in order to obtain the best conditions for analyzing these volatile compounds. Optimization of the working conditions for each of the different column combinations was performed by means of a central composite design. The best results in terms of separation and differentiation among the different chemical groups were achieved with a combination of inverted phase columns (first dimension: highly polar, INNOWax; second dimension: mid-polar, ZB-35). Additionally, a reference template was developed to provide an effective and rapid analysis of the target compounds. Finally, the proposed method was successfully employed to identify volatile compounds in raw and roasted almond samples from the Spanish cultivar Largueta"
Keywords:		Lythraceae/chemistry Mass Spectrometry Prunus/*chemistry Spain Volatile Organic Compounds/*analysis Almonds Central composite design Column selection Static headspace Volatile compounds;
Notes:		"MedlineManzano, Pilar Diego, Juan Carlos Bernal, Jose Luis Nozal, Maria Jesus Bernal, Jose eng Comparative Study Research Support, Non-U.S. Gov't Germany 2014/01/15 J Sep Sci. 2014 Mar; 37(6):675-83. doi: 10.1002/jssc.201301278. Epub 2014 Feb 8"