| Title: | "Glareosin: a novel sexually dimorphic urinary lipocalin in the bank vole, Myodes glareolus" |
| Author(s): | Loxley GM; Unsworth J; Turton MJ; Jebb A; Lilley KS; Simpson DM; Rigden DJ; Hurst JL; Beynon RJ; |
| Address: | "Centre for Proteome Research, Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB, UK. Mammalian Behaviour and Evolution Group, Institute of Integrative Biology, University of Liverpool, Leahurst Campus, Neston CH64 7TE, UK. Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB, UK. Department of Biochemistry, University of Leicester, Leicester LE1 7RH, UK. Centre for Proteome Research, Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB, UK r.beynon@liverpool.ac.uk" |
| ISSN/ISBN: | 2046-2441 (Electronic) 2046-2441 (Linking) |
| Abstract: | "The urine of bank voles (Myodes glareolus) contains substantial quantities of a small protein that is expressed at much higher levels in males than females, and at higher levels in males in the breeding season. This protein was purified and completely sequenced at the protein level by mass spectrometry. Leucine/isoleucine ambiguity was completely resolved by metabolic labelling, monitoring the incorporation of dietary deuterated leucine into specific sites in the protein. The predicted mass of the sequenced protein was exactly consonant with the mass of the protein measured in bank vole urine samples, correcting for the formation of two disulfide bonds. The sequence of the protein revealed that it was a lipocalin related to aphrodisin and other odorant-binding proteins (OBPs), but differed from all OBPs previously described. The pattern of secretion in urine used for scent marking by male bank voles, and the similarity to other lipocalins used as chemical signals in rodents, suggest that this protein plays a role in male sexual and/or competitive communication. We propose the name glareosin for this novel protein to reflect the origin of the protein and to emphasize the distinction from known OBPs" |
| Keywords: | "Amino Acid Sequence *Animal Communication Animals Arvicolinae/classification/*genetics Female Gene Expression Lipocalins/genetics/*isolation & purification/ultrastructure/urine Male Molecular Weight Pheromones/genetics Phylogeny Protein Conformation, alph;" |
| Notes: | "MedlineLoxley, Grace M Unsworth, Jennifer Turton, Michael J Jebb, Alexandra Lilley, Kathryn S Simpson, Deborah M Rigden, Daniel J Hurst, Jane L Beynon, Robert J eng BB/J002631/1/Biotechnology and Biological Sciences Research Council/United Kingdom BB/M012557/1/Biotechnology and Biological Sciences Research Council/United Kingdom Research Support, Non-U.S. Gov't England 2017/09/08 Open Biol. 2017 Sep; 7(9):170135. doi: 10.1098/rsob.170135" |
