| Title: | Cross-talk between high light stress and plant defence to the two-spotted spider mite in Arabidopsis thaliana |
| Author(s): | Barczak-Brzyzek AK; Kielkiewicz M; Gawronski P; Kot K; Filipecki M; Karpinska B; |
| Address: | "Warsaw University of Life Sciences - SGGW, Warsaw, Poland. Warsaw University of Life Sciences - SGGW, Warsaw, Poland. marcin_filipecki@sggw.pl. Centre for Plant Sciences, School of Biology, Faculty of Biological Sciences, University of Leeds, Leeds, UK" |
| DOI: | 10.1007/s10493-017-0187-x |
| ISSN/ISBN: | 1572-9702 (Electronic) 0168-8162 (Linking) |
| Abstract: | "Little is known about how plants deal with arthropod herbivores under the fluctuating light intensity and spectra which occur in natural environments. Moreover, the role of simultaneous stress such as excess light (EL) in the regulation of plant responses to herbivores is poorly characterized. In the current study, we focused on a mite-herbivore, specifically, the two-spotted spider mite (TSSM), which is one of the major agricultural pests worldwide. Our results showed that TSSM-induced leaf damage (visualized by trypan blue staining) and oviposition rate (measured as daily female fecundity) decreased after EL pre-treatment in wild-type Arabidopsis plants, but the observed responses were not wavelength specific. Thus, we established that EL pre-treatment reduced Arabidopsis susceptibility to TSSM infestation. Due to the fact that a portion of EL energy is dissipated by plants as heat in the mechanism known as non-photochemical quenching (NPQ) of chlorophyll fluorescence, we tested an Arabidopsis npq4-1 mutant impaired in NPQ. We showed that npq4-1 plants are significantly less susceptible to TSSM feeding activity, and this result was not dependent on light pre-treatment. Therefore, our findings strongly support the role of light in plant defence against TSSM, pointing to a key role for a photo-protective mechanism such as NPQ in this regulation. We hypothesize that plants impaired in NPQ are constantly primed to mite attack, as this seems to be a universal evolutionarily conserved mechanism for herbivores" |
| Keywords: | "Animals Arabidopsis/*physiology Female Fertility *Food Chain *Herbivory *Light Mutation Oviposition Plant Leaves/physiology Signal Transduction Stress, Physiological Tetranychidae/*physiology Biotic and abiotic stress interaction Excess light Non-photoche;" |
| Notes: | "MedlineBarczak-Brzyzek, A K Kielkiewicz, M Gawronski, P Kot, K Filipecki, M Karpinska, B eng REGPOT-2011-1-286093-WULS-Plant Health/FP7 Research Potential of Convergence Regions/ POKL.04.03.00-00-042/12-00/European Social Fund/ Netherlands 2017/11/10 Exp Appl Acarol. 2017 Oct; 73(2):177-189. doi: 10.1007/s10493-017-0187-x. Epub 2017 Nov 8" |
