| Title: | Lack of TRPM5-Expressing Microvillous Cells in Mouse Main Olfactory Epithelium Leads to Impaired Odor-Evoked Responses and Olfactory-Guided Behavior in a Challenging Chemical Environment |
| Author(s): | Lemons K; Fu Z; Aoude I; Ogura T; Sun J; Chang J; Mbonu K; Matsumoto I; Arakawa H; Lin W; |
| Address: | "Department of Biological Sciences, University of Maryland, Baltimore County, Baltimore, MD. Monell Chemical Senses Center, Philadelphia, PA. Department of Research Administration, School of Medicine, Case Western Reserve University, Cleveland, OH" |
| DOI: | 10.1523/ENEURO.0135-17.2017 |
| ISSN/ISBN: | 2373-2822 (Electronic) 2373-2822 (Linking) |
| Abstract: | "The mammalian main olfactory epithelium (MOE) modifies its activities in response to changes in the chemical environment. This process is essential for maintaining the functions of the olfactory system and the upper airway. However, mechanisms involved in this functional maintenance, especially those occurring via paracrine regulatory pathways within the multicellular MOE, are poorly understood. Previously, a population of non-neuronal, transient receptor potential M5-expressing microvillous cells (TRPM5-MCs) was identified in the MOE, and the initial characterization of these cells showed that they are cholinergic and responsive to various xenobiotics including odorants at high concentrations. Here, we investigated the role of TRPM5-MCs in maintaining olfactory function using transcription factor Skn-1a knockout (Skn-1a(-/-)) mice, which lack TRPM5-MCs in the MOE. Under our standard housing conditions, Skn-1a(-/-) mice do not differ significantly from control mice in odor-evoked electro-olfactogram (EOG) responses and olfactory-guided behaviors, including finding buried food and preference reactions to socially and sexually relevant odors. However, after a 2-wk exposure to high-concentration odor chemicals and chitin powder, Skn-1a(-/-) mice exhibited a significant reduction in their odor and pheromone-evoked EOG responses. Consequently, their olfactory-guided behaviors were impaired compared with vehicle-exposed Skn-1a(-/-) mice. Conversely, the chemical exposure did not induce significant changes in the EOG responses and olfactory behaviors of control mice. Therefore, our physiological and behavioral results indicate that TRPM5-MCs play a protective role in maintaining the olfactory function of the MOE" |
| Keywords: | "Animals Choice Behavior/physiology Evoked Potentials/genetics Feeding Behavior Female GAP-43 Protein/metabolism Gene Expression Regulation/drug effects/*genetics Male Maze Learning/physiology Mice Mice, Inbred C57BL Mice, Transgenic Octamer Transcription;" |
| Notes: | "MedlineLemons, Kayla Fu, Ziying Aoude, Imad Ogura, Tatsuya Sun, Julianna Chang, Justin Mbonu, Kenechukwu Matsumoto, Ichiro Arakawa, Hiroyuki Lin, Weihong eng R01 DC012831/DC/NIDCD NIH HHS/ R01 DC015491/DC/NIDCD NIH HHS/ R25 GM055036/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural 2017/06/15 eNeuro. 2017 Jun 12; 4(3):ENEURO.0135-17.2017. doi: 10.1523/ENEURO.0135-17.2017. eCollection 2017 May-Jun" |
