| Title: | "Molecular characterization of SIG1, a Saccharomyces cerevisiae gene involved in negative regulation of G-protein-mediated signal transduction" |
| Author(s): | Leberer E; Dignard D; Harcus D; Whiteway M; Thomas DY; |
| Address: | "Biotechnology Research Institute, National Research of Canada, Montreal, Quebec" |
| DOI: | 10.1002/j.1460-2075.1994.tb06604.x |
| ISSN/ISBN: | 0261-4189 (Print) 1460-2075 (Electronic) 0261-4189 (Linking) |
| Abstract: | "Two recessive mutations in the Saccharomyces cerevisiae SIG1 (suppressor of inhibitory G-protein) gene have been identified by their ability to suppress the signalling defect of dominant-negative variants of the mating response G-protein beta-subunit. The mutations and deletion of SIG1 enhance the sensitivity of the cells to pheromone and stimulate the basal transcription of a mating specific gene, FUS1, suggesting that Sig1p plays a negatively regulatory role in G beta gamma-mediated signal transduction. An additional function of Sig1p in vegetatively growing cells is suggested by the finding that the mutations and deletion of SIG1 cause temperature-sensitive growth defects. The SIG1 gene encodes a protein with a molecular weight of 65 kDa that contains at the amino-terminus two zinc finger-like sequence motifs. Epistasis experiments localize the action of Sig1p within the pheromone signalling pathway at a position at or shortly after the G-protein. We propose that Sig1p represents a novel negative regulator of G beta gamma-mediated signal transduction" |
| Keywords: | "Amino Acid Sequence Base Sequence Chromosome Mapping Cloning, Molecular DNA, Fungal Fungal Proteins/*genetics/metabolism/physiology GTP-Binding Proteins/*metabolism Gene Expression Regulation, Fungal/genetics Genes, Fungal Mating Factor Membrane Proteins;" |
| Notes: | "MedlineLeberer, E Dignard, D Harcus, D Whiteway, M Thomas, D Y eng Research Support, Non-U.S. Gov't England 1994/07/01 EMBO J. 1994 Jul 1; 13(13):3050-64. doi: 10.1002/j.1460-2075.1994.tb06604.x" |
