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Biosens Bioelectron

Title:		Determination of the nanoscale electrical properties of olfactory receptor hOR1A1 and their dependence on ligand binding: Towards the development of capacitance-operated odorant biosensors
Author(s):		Lagunas A; Belloir C; Briand L; Gorostiza P; Samitier J;
Address:		"Biomedical Research Networking Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), av. Monforte de Lemos, 3-5. Pabellon 11. Planta 0, 28029, Madrid, Spain; Institute for Bioengineering of Catalonia (IBEC), Barcelona Institute of Science and Technology, c/Baldiri i Reixac 10-12, 08028, Barcelona, Spain. Electronic address: alagunas@ibecbarcelona.eu. Centre des Sciences du Gout et de l'Alimentation, CNRS, INRAE, Institut Agro Dijon, Universite Bourgogne Franche-Comte, 9E Bd Jeanne d'Arc, 21000, Dijon, France. Biomedical Research Networking Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), av. Monforte de Lemos, 3-5. Pabellon 11. Planta 0, 28029, Madrid, Spain; Institute for Bioengineering of Catalonia (IBEC), Barcelona Institute of Science and Technology, c/Baldiri i Reixac 10-12, 08028, Barcelona, Spain; Catalan Institution for Research and Advanced Studies (ICREA), pg. Lluis Companys 23, Barcelona, 08010, Spain. Biomedical Research Networking Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), av. Monforte de Lemos, 3-5. Pabellon 11. Planta 0, 28029, Madrid, Spain; Institute for Bioengineering of Catalonia (IBEC), Barcelona Institute of Science and Technology, c/Baldiri i Reixac 10-12, 08028, Barcelona, Spain; Department of Electronics and Biomedical Engineering, Faculty of Physics, University of Barcelona (UB), c/Marti i Franques 1, 08028, Barcelona, Spain. Electronic address: jsamitier@ibecbarcelona.eu"
Journal Title:		Biosens Bioelectron
Year:		2022
Volume:		20220928
Issue:
Page Number:		114755 -
DOI:		10.1016/j.bios.2022.114755
ISSN/ISBN:		1873-4235 (Electronic) 0956-5663 (Linking)
Abstract:		"The transduction of odorant binding into cellular signaling by olfactory receptors (ORs) is not understood and knowing its mechanism would enable developing new pharmacology and biohybrid electronic detectors of volatile organic compounds bearing high sensitivity and selectivity. The electrical characterization of ORs in bulk experiments is subject to microscopic models and assumptions. We have directly determined the nanoscale electrical properties of ORs immobilized in a fixed orientation, and their change upon odorant binding, using electrochemical scanning tunneling microscopy (EC-STM) in near-physiological conditions. Recordings of current versus time, distance, and electrochemical potential allows determining the OR impedance parameters and their dependence with odorant binding. Our results allow validating OR structural-electrostatic models and their functional activation processes, and anticipating a novel macroscopic biosensor based on ORs"
Keywords:		"*Biosensing Techniques Ligands Odorants *Receptors, Odorant/chemistry *Volatile Organic Compounds/chemistry Electrochemical scanning tunneling microscopy (EC-STM) Odorant binding Olfactory receptors (ORs) Open-circuit voltage (V(OC));"
Notes:		"MedlineLagunas, Anna Belloir, Christine Briand, Loic Gorostiza, Pau Samitier, Josep eng England 2022/10/04 Biosens Bioelectron. 2022 Dec 15; 218:114755. doi: 10.1016/j.bios.2022.114755. Epub 2022 Sep 28"