| Title: | "Molecular characterization of diapause hormone and pheromone biosynthesis activating neuropeptide from the black-back prominent moth, Clostera anastomosis (L.) (Lepidoptera, Notodontidae)" |
| Author(s): | Jing TZ; Wang ZY; Qi FH; Liu KY; |
| Address: | "School of Forestry, Northeast Forestry University, Harbin 150040, China. jingtianzhong@yahoo.com" |
| DOI: | 10.1016/j.ibmb.2007.07.012 |
| ISSN/ISBN: | 0965-1748 (Print) 0965-1748 (Linking) |
| Abstract: | "Using a strategy of rapid amplification of cDNA ends, the cDNA of diapause hormone (DH) and pheromone biosynthesis activating neuropeptide (PBAN) was cloned from the head of Clostera anastomosis (L.). The Cloan-DH-PBAN cDNA contains an open reading frame encoding a 196-amino acid preprohormone, from which five putative FXPRL peptides, DH, PBAN, alpha-SGNP(SGNP, suboesophageal ganglion neuropeptide), beta-SGNP and gamma-SGNP, are released. Comparing the deduced amino acid sequences from cDNAs of these five FXPRL peptides to those known from other insects, Cloan-DH shows highest similarity of 93.1% to that from Agrotis ipsilon, Cloan-PBAN 93.9% to those from Helicoverpa armigera, Helicoverpa zea and Helicoverpa assulta, which show the highest similarity to species of Noctuidae. Phylogenetic analysis revealed that the Cloan-DH-PBAN gene is relatively closely related to those from Noctuoidea, but distant from those from Tortricoidea, Yponomeutoidea and Bombycoidea species. The DNA sequence encoding Cloan-DH-PBAN was cloned by PCR, which is 3698bp in size and comprises six exons interspersed by five introns. Developmental expression of the DH-PBAN transcripts in the head was also showed by a semi-quantitative RT-PCR method, which was relatively low in larvae and remained low in pupae of both sexes, increased sharply in adults of both sexes" |
| Keywords: | "Amino Acid Sequence Animals Base Sequence Biological Evolution Female Gene Expression Regulation, Developmental Genes, Insect Male Molecular Sequence Data Molecular Structure Moths/*chemistry/genetics/metabolism Multigene Family Neuropeptides/*chemistry/g;" |
| Notes: | "MedlineJing, Tian-Zhong Wang, Zhi-Ying Qi, Feng-Hui Liu, Kuan-Yu eng Research Support, Non-U.S. Gov't England 2007/10/31 Insect Biochem Mol Biol. 2007 Dec; 37(12):1262-71. doi: 10.1016/j.ibmb.2007.07.012. Epub 2007 Aug 6" |
