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Methods Mol Biol

Title:		"Acute behavioral responses to pheromones in C. elegans (adult behaviors: attraction, repulsion)"
Author(s):		Jang H; Bargmann CI;
Address:		"The Rockefeller University, New York, NY, USA"
Journal Title:		Methods Mol Biol
Year:		2013
Volume:		1068
Issue:
Page Number:		285 - 292
DOI:		10.1007/978-1-62703-619-1_21
ISSN/ISBN:		1940-6029 (Electronic) 1064-3745 (Linking)
Abstract:		"The pheromone drop test is a simple and robust behavioral assay to quantify acute avoidance of pheromones in C. elegans, and the suppression of avoidance by attractive pheromones. In the pheromone drop test, water-soluble C. elegans pheromones are individually applied to animals that are freely moving on a large plate. Upon encountering a repellent, each C. elegans animal may or may not try to escape by making a long reversal. The fraction of animals that make a long reversal response indicates the repulsiveness of a given pheromone to a specific genotype/strain of C. elegans. Performing the drop test in the presence of bacterial food enhances the avoidance response to pheromones. Attraction to pheromones can be assayed by the suppression of reversals to repulsive pheromones or by the suppression of the basal reversal rate to buffer"
Keywords:		"Animals Behavior, Animal/*drug effects/physiology Caenorhabditis elegans/*drug effects/physiology Caenorhabditis elegans Proteins/metabolism/pharmacology Chemotaxis/*drug effects Locomotion/*drug effects Motor Activity/drug effects Pheromones/metabolism/*;"
Notes:		"MedlineJang, Heeun Bargmann, Cornelia I eng 2013/09/10 Methods Mol Biol. 2013; 1068:285-92. doi: 10.1007/978-1-62703-619-1_21"