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Int J Food Microbiol

Title:		Incidence of virulence factors in enterococci from raw and fermented meat and biofilm forming capacity at 25 degrees C and 37 degrees C
Author(s):		Jahan M; Holley RA;
Address:		"Department of Food Science, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada. Department of Food Science, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada. Electronic address: rick_holley@umanitoba.ca"
Journal Title:		Int J Food Microbiol
Year:		2014
Volume:		20131110
Issue:
Page Number:		65 - 69
DOI:		10.1016/j.ijfoodmicro.2013.11.002
ISSN/ISBN:		1879-3460 (Electronic) 0168-1605 (Linking)
Abstract:		"Twenty-nine Enterococcus strains from raw and fermented meat products were screened for the presence of virulence genes, including those for aggregation substances (asa1 and asa373), cytolysin activator (cylA), collagen binding protein (ace), endocarditis antigen (efaA), enterococcal surface protein (esp) and gelatinase (gelE). Virulence gene occurrence, expression of gelatinase and pheromone aggregation was greater in Enterococcus faecalis than in Enterococcus faecium strains. All E. faecalis and 54% of E. faecium were positive for at least one or more virulence gene. The only strain of Enterococcus gallinarum tested also contained virulence genes. The effect of different growth temperatures (25 and 37 degrees C) on biofilm formation using polystyrene plates was also assessed. Strong biofilm formation occurred at lower than optimum temperature in all three species of enterococci. Neither esp nor gelE was necessary for biofilm formation and this relationship was species rather than strain specific. This study emphasizes the importance of enterococci as a reservoir of virulence genes and the potential for their genetic transfer to human strains following consumption of uncooked or undercooked contaminated meat"
Keywords:		Biofilms/*growth & development Enterococcus/drug effects/genetics/pathogenicity/*physiology *Fermentation *Food Microbiology/statistics & numerical data Humans Meat/*microbiology *Temperature Virulence Factors/genetics Biofilm formation Enterococci Subopt;
Notes:		"MedlineJahan, Musarrat Holley, Richard A eng Research Support, Non-U.S. Gov't Netherlands 2013/12/03 Int J Food Microbiol. 2014 Jan 17; 170:65-9. doi: 10.1016/j.ijfoodmicro.2013.11.002. Epub 2013 Nov 10"