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Environ Toxicol

Title:		Identification of time-dependent biomarkers and effects of exposure to volatile organic compounds using high-throughput analysis
Author(s):		Hong JY; Yu SY; Kim GW; Ahn JJ; Kim Y; Lim S; Son SW; Hwang SY;
Address:		"Department of Bio-Nanotechnology, Hanyang University, Sangnok-Gu, Ansan, Gyeonggi-Do, Korea. Department of Molecular & Life Science, Hanyang University, Sangnok-Gu, Ansan, Gyeonggi-Do, Korea. Department of Dermatology, Korea University Medical Center, Seoul, South Korea. Department of Bio-Nanotechnology, Hanyang University, Sangnok-Gu, Ansan, Gyeonggi-Do, Korea. syhwang@hanyang.ac.kr. Department of Molecular & Life Science, Hanyang University, Sangnok-Gu, Ansan, Gyeonggi-Do, Korea. syhwang@hanyang.ac.kr"
Journal Title:		Environ Toxicol
Year:		2016
Volume:		20150528
Issue:		11
Page Number:		1563 - 1570
DOI:		10.1002/tox.22160
ISSN/ISBN:		1522-7278 (Electronic) 1520-4081 (Linking)
Abstract:		"Volatile organic compounds (VOCs) can be easily taken up by humans, leading to various diseases, such as respiratory system and central nervous system disorders. Environmental risk assessment is generally conducted using traditional tests, which may be time-consuming and technically challenging. Therefore, analysis of the effects of VOCs, such as toluene, ethylbenzene, and xylene, may be improved by use of novel, high-throughput methods, such as microarray analysis. In this study, we examined the effects of VOCs exposure in humans on gene expression and methylation using microarray analysis. We recruited participants who had short-term exposure, long-term exposure, or no exposure. We then analyzed changes in gene expression in blood samples from these participants. A total of 866 genes were upregulated, while 366 genes were downregulated in the short-term exposure group. Similarly, in the long-term exposure group, a total of 852 and 480 genes were up- or downregulated, respectively. Hierarchical clustering analysis was used to divide the clustered genes into nine clusters to investigate the expression of variations in accordance with the exposure period. And the methylation microarray was performed at the same time to see whether this expression variation is related to the epigenetic study. Finally, we have 5 genes that were upregulated and 12 genes that were downregulated, gradually and respectively, so these genes are expected to function as biomarkers of the duration of exposure to VOCs. Further research is required to determine the time-dependent effects of VOCs on epigenetic regulation of gene expression. (c) 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1563-1570, 2016"
Keywords:		"Biomarkers/analysis Environmental Pollutants/*toxicity Epigenesis, Genetic/drug effects Gene Expression Regulation/drug effects High-Throughput Screening Assays Humans Male Oligonucleotide Array Sequence Analysis Time Factors Volatile Organic Compounds/*t;"
Notes:		"MedlineHong, Ji Young Yu, So Yeon Kim, Gi Won Ahn, Jeong Jin Kim, Youngjoo Lim, Seri Son, Sang Wook Hwang, Seung Yong eng 2016/10/19 Environ Toxicol. 2016 Nov; 31(11):1563-1570. doi: 10.1002/tox.22160. Epub 2015 May 28"