| Title: | High-throughput log P determination by MEEKC coupled with UV and MS detections |
| Author(s): | Henchoz Y; Romand S; Schappler J; Rudaz S; Veuthey JL; Carrupt PA; |
| Address: | "School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Quai Ernest-Ansermet, Geneva, Switzerland" |
| ISSN/ISBN: | 1522-2683 (Electronic) 0173-0835 (Linking) |
| Abstract: | "A high-throughput screening method using MEEKC was developed for the determination of 1-octanol-water partition coefficients (log P(oct)). Two approaches were carried out to decrease determination times to about 20 min per compound: (i) a dynamically coated capillary was used to increase the EOF at low pH, allowing the measurement of log P(oct) of acidic compounds and (ii) a short-end injection was performed to reduce the capillary effective length. The analytical conditions were optimized to determine the lipophilicity of neutral, basic, and acidic compounds with log P(oct) ranging from 0 to 5. The developed method was first applied to a well-balanced set of 35 reference compounds, and second to a set of 21 acidic and 29 basic pharmaceutical compounds. Finally, determinations were achieved with MS detection, allowing a 20-fold throughput increase thanks to sample pooling. An atmospheric pressure photoionization source was selected to advantageously replace ESI as it was less affected by the non-volatile BGE additives used in MEEKC" |
| Keywords: | "Calibration Chromatography, Micellar Electrokinetic Capillary/*methods Emulsions/*chemistry Least-Squares Analysis Mass Spectrometry/*methods Organic Chemicals/chemistry Pharmaceutical Preparations/chemistry Solvents/chemistry;" |
| Notes: | "MedlineHenchoz, Yveline Romand, Stephanie Schappler, Julie Rudaz, Serge Veuthey, Jean-Luc Carrupt, Pierre-Alain eng Germany 2010/03/02 Electrophoresis. 2010 Mar; 31(5):952-64. doi: 10.1002/elps.200900540" |
