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G3 (Bethesda)

Title:		Draft genome sequences of strains CBS6241 and CBS6242 of the basidiomycetous yeast Filobasidium floriforme
Author(s):		Guerreiro MA; Ahrendt S; Pangilinan J; Chen C; Yan M; Lipzen A; Barry K; Grigoriev IV; Begerow D; Nowrousian M;
Address:		"Department of Evolution of Plants and Fungi, Ruhr-Universitat Bochum, Bochum 44801, Germany. U.S. Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Department of Plant and Microbial Biology, University of California Berkeley, Berkeley, CA 94720, USA. Department of Molecular and Cellular Botany, Ruhr-Universitat Bochum, Bochum 44801, Germany"
Journal Title:		G3 (Bethesda)
Year:		2022
Volume:		12
Issue:		2
Page Number:		-
DOI:		10.1093/g3journal/jkab398
ISSN/ISBN:		2160-1836 (Electronic) 2160-1836 (Linking)
Abstract:		"The Tremellomycetes are a species-rich group within the basidiomycete fungi; however, most analyses of this group to date have focused on pathogenic Cryptococcus species within the order Tremellales. Recent genome-assisted studies of other Tremellomycetes have identified interesting features with respect to biotechnological applications as well as the evolution of genes involved in mating and sexual development. Here, we report genome sequences of two strains of Filobasidium floriforme, a species from the order Filobasidiales, which branches basally to the Tremellales, Trichosporonales, and Holtermanniales. The assembled genomes of strains CBS6241 and CBS6242 are 27.4 Mb and 26.4 Mb in size, respectively, with 8314 and 7695 predicted protein-coding genes. Overall sequence identity at nucleic acid level between the strains is 97%. Among the predicted genes are pheromone precursor and pheromone receptor genes as well as two genes encoding homedomain (HD) transcription factors, which are predicted to be part of the mating type (MAT) locus. Sequence analysis indicates that CBS6241 and CBS6242 carry different alleles for both the pheromone/receptor genes as well as the HD transcription factors. Orthology inference identified 1482 orthogroups exclusively found in F. floriforme, some of which were involved in carbohydrate transport and metabolism. Subsequent CAZyme repertoire characterization identified 267 and 247 enzymes for CBS6241 and CBS6242, respectively, the second highest number of CAZymes among the analyzed Tremellomycete species. In addition, F. floriforme contains five CAZymes absent in other species and several plant-cell-wall degrading CAZymes with the highest copy number in Tremellomycota, indicating the biotechnological potential of this species"
Keywords:		"*Basidiomycota/genetics DNA, Fungal/genetics Genes, Mating Type, Fungal Phylogeny Receptors, Pheromone/genetics Filobasidium floriforme CAZymes Filobasidiales basidiomycete mating-type locus;"
Notes:		"MedlineGuerreiro, Marco Alexandre Ahrendt, Steven Pangilinan, Jasmyn Chen, Cindy Yan, Mi Lipzen, Anna Barry, Kerrie Grigoriev, Igor V Begerow, Dominik Nowrousian, Minou eng Research Support, Non-U.S. Gov't England 2021/11/19 G3 (Bethesda). 2022 Feb 4; 12(2):jkab398. doi: 10.1093/g3journal/jkab398"