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Int J Biol Macromol

Title:		DORN1 and GORK regulate stomatal closure in Arabidopsis mediated by volatile organic compound ethyl vinyl ketone
Author(s):		Gong J; Wang Z; Guo Z; Yao L; Zhao C; Lin S; Ma S; Shen Y;
Address:		"National Engineering Research Center of Tree breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Beijing 100083, PR China. National Engineering Research Center of Tree breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Beijing 100083, PR China. Electronic address: Alice0521@bjfu.edu.cn. Beijing Jingtai Technology Co., Ltd., Beijing 100083, PR China. Electronic address: chuangfang.zhao@xtalpi.com. Beijing Jingtai Technology Co., Ltd., Beijing 100083, PR China. Electronic address: sheng.lin@xtalpi.com. Beijing Jingtai Technology Co., Ltd., Beijing 100083, PR China. Electronic address: songling.ma@xtalpi.com. National Engineering Research Center of Tree breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Beijing 100083, PR China. Electronic address: ybshen@bjfu.edu.cn"
Journal Title:		Int J Biol Macromol
Year:		2023
Volume:		20230201
Issue:
Page Number:		123503 -
DOI:		10.1016/j.ijbiomac.2023.123503
ISSN/ISBN:		1879-0003 (Electronic) 0141-8130 (Linking)
Abstract:		"Evk (ethyl vinyl ketone) is a signal substance for plant defense, but little is known about how evk mediates stomatal closure. Through stomatal biology experiments, we found that evk can mediate stomatal closure, and stomatal closure is weakened when DORN1 (DOES NOT RESPOND TO NUCLEOTIDES 1) and GORK (GATED OUTWARDLY-RECTIFYING K+ CHANNEL) are mutated. In addition, it was found by non-invasive micro-test technology (NMT) that the K(+) efflux mediated by evk was significantly weakened when DORN and GORK were mutated. Yeast two-hybrid (Y2H), firefly luciferase complementation imaging (LCI), and in vitro pull-down assays demonstrated that DORN1 and GORK could interact in vitro and in vivo. It was found by molecular docking that evk could combine with MRP (Multidrug Resistance-associated Protein), thus affecting ATP transport, promoting eATP (extracellular ATP) concentration increase and realizing downstream signal transduction. Through inoculation of botrytis cinerea, it was found that evk improved the antibacterial activity of Arabidopsis thaliana. As revealed by reverse transcription quantitative PCR (RT-qPCR), the expression of defense related genes was enhanced by evk treatment. Evk is a potential green antibacterial drug"
Keywords:		*Arabidopsis/metabolism *Volatile Organic Compounds Molecular Docking Simulation Potassium Channels/genetics *Arabidopsis Proteins/metabolism Adenosine Triphosphate/metabolism Dorn1 Ethyl vinyl ketone Gork Stomatal closure;
Notes:		"MedlineGong, Junqing Wang, Zhaoyuan Guo, Zhujuan Yao, Lijuan Zhao, Chuanfang Lin, Sheng Ma, Songling Shen, Yingbai eng Netherlands 2023/02/04 Int J Biol Macromol. 2023 Mar 15; 231:123503. doi: 10.1016/j.ijbiomac.2023.123503. Epub 2023 Feb 1"