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Phytochemistry

Title:		Differential metabolism of L-phenylalanine in the formation of aromatic volatiles in melon (Cucumis melo L.) fruit
Author(s):		Gonda I; Davidovich-Rikanati R; Bar E; Lev S; Jhirad P; Meshulam Y; Wissotsky G; Portnoy V; Burger J; Schaffer AA; Tadmor Y; Giovannoni JJ; Fei Z; Fait A; Katzir N; Lewinsohn E;
Address:		"Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, The Volcani Center, P.O. Box 1021, Ramat Yishay, 30095, Israel; The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Beer-Sheva, 84105, Israel. Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, The Volcani Center, P.O. Box 1021, Ramat Yishay, 30095, Israel. Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, The Volcani Center, P.O. Box 1021, Ramat Yishay, 30095, Israel; ORT Braude College, Department of Biotechnology Engineering, Karmiel, Israel. Institute of Plant Sciences, The Volcani Center, Agricultural Research Organization, P.O. Box 6, Rishon LeZion, 76100, Israel. The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Beer-Sheva, 84105, Israel. Boyce Thompson Institute, Cornell University, Ithaca, NY 14853, USA; USDA-ARS Robert W. Holley Center for Agriculture and Health, Ithaca, NY 14853, USA. Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, The Volcani Center, P.O. Box 1021, Ramat Yishay, 30095, Israel. Electronic address: twefraim@agri.gov.il"
Journal Title:		Phytochemistry
Year:		2018
Volume:		20180220
Issue:
Page Number:		122 - 131
DOI:		10.1016/j.phytochem.2017.12.018
ISSN/ISBN:		1873-3700 (Electronic) 0031-9422 (Linking)
Abstract:		"Studies on the active pathways and the genes involved in the biosynthesis of L-phenylalanine-derived volatiles in fleshy fruits are sparse. Melon fruit rinds converted stable-isotope labeled L-phe into more than 20 volatiles. Phenylpropanes, phenylpropenes and benzenoids are apparently produced via the well-known phenylpropanoid pathway involving phenylalanine ammonia lyase (PAL) and being (E)-cinnamic acid a key intermediate. Phenethyl derivatives seemed to be derived from L-phe via a separate biosynthetic route not involving (E)-cinnamic acid and PAL. To explore for a biosynthetic route to (E)-cinnamaldehyde in melon rinds, soluble protein cell-free extracts were assayed with (E)-cinnamic acid, CoA, ATP, NADPH and MgSO(4), producing (E)-cinnamaldehyde in vitro. In this context, we characterized CmCNL, a gene encoding for (E)-cinnamic acid:coenzyme A ligase, inferred to be involved in the biosynthesis of (E)-cinnamaldehyde. Additionally we describe CmBAMT, a SABATH gene family member encoding a benzoic acid:S-adenosyl-L-methionine carboxyl methyltransferase having a role in the accumulation of methyl benzoate. Our approach leads to a more comprehensive understanding of L-phe metabolism into aromatic volatiles in melon fruit"
Keywords:		"Cucumis melo/*chemistry Fruit/*chemistry Glucosides/chemistry/isolation & purification Glycosylation Methionine/metabolism Nuclear Magnetic Resonance, Biomolecular Phenylalanine/*metabolism Phenylalanine Ammonia-Lyase/genetics Plant Proteins/metabolism S-;"
Notes:		"MedlineGonda, Itay Davidovich-Rikanati, Rachel Bar, Einat Lev, Shery Jhirad, Pliaa Meshulam, Yuval Wissotsky, Guy Portnoy, Vitaly Burger, Joseph Schaffer, Arthur A Tadmor, Yaakov Giovannoni, James J Fei, Zhangjun Fait, Aaron Katzir, Nurit Lewinsohn, Efraim eng England 2018/02/16 Phytochemistry. 2018 Apr; 148:122-131. doi: 10.1016/j.phytochem.2017.12.018. Epub 2018 Feb 20"