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Plant Cell

Title:		Functional analysis of the 3' control region of the potato wound-inducible proteinase inhibitor II gene
Author(s):		An G; Mitra A; Choi HK; Costa MA; An K; Thornburg RW; Ryan CA;
Address:		"Institute of Biological Chemistry, Washington State University, Pullman 99164-6340"
Journal Title:		Plant Cell
Year:		1989
Volume:		1
Issue:		1
Page Number:		115 - 122
DOI:		10.1105/tpc.1.1.115
ISSN/ISBN:		1040-4651 (Print) 1532-298X (Electronic) 1040-4651 (Linking)
Abstract:		"Proteinase inhibitor genes are expressed strongly in specific plant tissues under both developmental and environmental regulation. We have studied the role of the 3' control region of the potato proteinase inhibitor II gene (PI-II) that is inducible in leaves in response to herbivore attacks or other severe wounding. Comparison of the terminator from the PI-II gene with two different terminators from the 6b and 7 genes, driven by a common PI-II promoter-cat fusion molecule, indicated that the PI-II terminator provided the most efficient expression of cat. The PI-II terminator also caused a significantly elevated cat gene expression driven by the cauliflower mosaic virus 35S promoter. The increase in the level of expression is probably not due to the presence of an enhancer element in the PI-II terminator region, but to cis-acting elements involved in mRNA processing or stability. Both transient and stable transformation analyses of the deletion mutants in the 3'-flanking sequence indicated that about a 100-base pair DNA fragment surrounding the polyadenylation site is essential for the efficient gene expression. This region seems to consist of several regulatory elements, including the conserved sequence, CGTGTCTT, which is located 9 bases downstream from the polyadenylation site. The elements appear to contribute to the increased stability of mRNAs containing the PI-II terminator"
Keywords:		"Amino Acid Sequence Base Sequence DNA Gene Expression Regulation Molecular Sequence Data Promoter Regions, Genetic *Protease Inhibitors RNA, Messenger/metabolism *Regulatory Sequences, Nucleic Acid Solanum tuberosum/*genetics Terminator Regions, Genetic;"
Notes:		"MedlineAn, G Mitra, A Choi, H K Costa, M A An, K Thornburg, R W Ryan, C A eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 1989/01/01 Plant Cell. 1989 Jan; 1(1):115-22. doi: 10.1105/tpc.1.1.115"