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J Pharm Biomed Anal

Title:		Coupling non-denaturing chromatography to mass spectrometry for the characterization of monoclonal antibodies and related products
Author(s):		Farsang E; Guillarme D; Veuthey JL; Beck A; Lauber M; Schmudlach A; Fekete S;
Address:		"Department of Analytical Chemistry, University of Pannonia, Egyetem u. 10., H-8200 Veszprem, Hungary. Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, CMU-Rue Michel Servet 1, 1211, Geneva 4, Switzerland. Center of Immunology Pierre Fabre, 5 Avenue Napoleon III, BP 60497, 74160, Saint-Julien-en-Genevois, France. Waters Corporation, 34 Maple Street, Milford, MA, 01757-3696, United States. Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, CMU-Rue Michel Servet 1, 1211, Geneva 4, Switzerland. Electronic address: szabolcs.fekete@unige.ch"
Journal Title:		J Pharm Biomed Anal
Year:		2020
Volume:		20200225
Issue:
Page Number:		113207 -
DOI:		10.1016/j.jpba.2020.113207
ISSN/ISBN:		1873-264X (Electronic) 0731-7085 (Linking)
Abstract:		"The hyphenation of non-denaturing liquid chromatographic (LC) modes (ion exchange (IEX), size exclusion (SEC) and hydrophobic interaction chromatography (HIC)) with mass spectrometry (MS) has attracted significant attention in the last few years. The inherent problem of these couplings is that non-denaturing LC separations have tended to use non-volatile mobile phase additives. Indeed, classical methods have not been directly compatible with MS. Therefore two approaches can be used to address this challenge: (1) finding innovative volatile mobile phases or (2) adding a desalting step prior to MS detection via the use of multidimensional LC. These two possibilities have been applied to the characterization of charge-, size- and hydrophobic variants of various monoclonal antibodies (mAbs) and related products and have been reviewed in this paper"
Keywords:		"Antibodies, Monoclonal/*analysis/chemistry Buffers Chemistry, Pharmaceutical/*methods Chromatography, Gel/methods Chromatography, Ion Exchange/methods Hydrophobic and Hydrophilic Interactions Mass Spectrometry/*methods Proteolysis Volatile Organic Compoun;"
Notes:		"MedlineFarsang, Evelin Guillarme, Davy Veuthey, Jean-Luc Beck, Alain Lauber, Matthew Schmudlach, Andrew Fekete, Szabolcs eng Review England 2020/03/07 J Pharm Biomed Anal. 2020 Jun 5; 185:113207. doi: 10.1016/j.jpba.2020.113207. Epub 2020 Feb 25"