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J Insect Physiol

Title:		Identification of an intraspecific alarm pheromone and two conserved odorant-binding proteins associated with (E)-beta-farnesene perception in aphid Rhopalosiphum padi
Author(s):		Fan J; Xue W; Duan H; Jiang X; Zhang Y; Yu W; Jiang S; Sun J; Chen J;
Address:		"State Key Laboratory of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China. State Key Laboratory of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: jlchen@ippcaas.cn"
Journal Title:		J Insect Physiol
Year:		2017
Volume:		20170801
Issue:
Page Number:		151 - 160
DOI:		10.1016/j.jinsphys.2017.07.014
ISSN/ISBN:		1879-1611 (Electronic) 0022-1910 (Linking)
Abstract:		"(E)-beta-farnesene (EBF) is the common active component of aphid alarm pheromone. Either or both of two orthologs of ordorant-binding proteins (OBPs), OBP3 and OBP7, recently reported in aphids, may be involved in EBF perception. The aim of this study was to investigate the respondence of the aphid Rhopalosiphum padi to its intraspecific alarm pheromone and which OBP is responsible for that response. We tested the olfactory response of the aphid R. padi to EBF and freshly crushed aphids. Then, we extracted the volatiles from crushed aphids using solid phase microextraction (SPME) for analysis with GCxGC-TOF/MS. We also cloned two OBPs cDNAs in R. padi (RpadOBP3 and RpadOBP7) and expressed them in competent Escherichia coli cells. Both recombinant proteins, RpadOBP3 and RpadOBP7, bound EBF well, with RpadOBP7 having specifically stronger affinity for EBF than for other volatiles. Based on the crystal structure of the OBPs with high identity, we performed homology modeling and analyzed the interactions between RpadOBPs and EBF. In conclusion, R. padi was repelled by both EBF and crushed aphids. EBF was identified as the only volatile that acted as the alarm pheromone. Our results indicated that OBP7 is a potential molecular target to control wheat aphids by disturbing their behaviors to the alarm pheromone"
Keywords:		"Amino Acid Sequence Animals Aphids/*genetics/growth & development/*metabolism Cloning, Molecular DNA, Complementary/genetics/metabolism Insect Proteins/chemistry/*genetics/metabolism Nymph/genetics/growth & development/metabolism Pheromones/*metabolism RN;"
Notes:		"MedlineFan, Jia Xue, Wenxin Duan, Hongxia Jiang, Xin Zhang, Yong Yu, Wenjuan Jiang, Shanshan Sun, Jingrun Chen, Julian eng Research Support, Non-U.S. Gov't England 2017/08/06 J Insect Physiol. 2017 Aug; 101:151-160. doi: 10.1016/j.jinsphys.2017.07.014. Epub 2017 Aug 1"