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Food Chem

Title:		Surface characteristics and proteomic analysis insights on the response of Oenococcus oeni SD-2a to freeze-drying stress
Author(s):		Yang K; Liu M; Wang J; Hassan H; Zhang J; Qi Y; Wei X; Fan M; Zhang G;
Address:		"College of Food Science and Engineering, Northwest A & F University, Yangling 712100, China; XiZang Agriculture and Animal Husbandry College, Linzhi 860000, China. College of Food Science and Engineering, Northwest A & F University, Yangling 712100, China. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China. College of Food Science and Engineering, Northwest A & F University, Yangling 712100, China. Electronic address: fanmt@nwsuaf.edu.cn. XiZang Agriculture and Animal Husbandry College, Linzhi 860000, China. Electronic address: guoqiang2008@163.com"
Journal Title:		Food Chem
Year:		2018
Volume:		20180502
Issue:
Page Number:		377 - 385
DOI:		10.1016/j.foodchem.2018.04.137
ISSN/ISBN:		1873-7072 (Electronic) 0308-8146 (Linking)
Abstract:		"Oenococcus oeni (O. oeni) induces malolactic fermentation to improve wine quality. However, the molecular basis of mechanisms involved in its freeze-drying resistance remains unclear. In this work, O. oeni SD-2a without freeze-drying (No-FD), with freeze-drying (FD) and freeze-drying with monosodium glutamate (MSG) (FD-MSG) were investigated. Flow cytometry results showed severe cell damage in FD cells and less membrane transmissibility in FD-MSG cells. Meanwhile, extracellular polymeric substances (EPS) were detected in FD and FD-MSG cells by scanning electron microscopy. Bioinformatic analysis revealed that varying proteins were involved in carbon, lipid and nucleic acids metabolism, stress response, oxidoreductase activity and signal sensing. Among the identified proteins, the highlighted proteins were those involved in polysaccharides production and signal sensing for freeze-drying resistance and cyclopropane fatty acid metabolism for MSG addition"
Keywords:		"Bacterial Proteins/analysis/*metabolism Carbohydrate Metabolism Cell Wall/chemistry Electrophoresis, Gel, Two-Dimensional *Freeze Drying Microscopy, Electron, Scanning Oenococcus/chemistry/*metabolism Oxidoreductases/metabolism *Proteomics/methods Quorum;"
Notes:		"MedlineYang, Kun Liu, Miaomiao Wang, Jing Hassan, Hamada Zhang, Jie Qi, Yiman Wei, Xinyuan Fan, Mingtao Zhang, Guoqiang eng England 2018/06/02 Food Chem. 2018 Oct 30; 264:377-385. doi: 10.1016/j.foodchem.2018.04.137. Epub 2018 May 2"