| Title: | Expressional divergence of the fatty acid-amino acid conjugate-hydrolyzing aminoacylase 1 (L-ACY-1) in Helicoverpa armigera and Helicoverpa assulta |
| Author(s): | Cheng Q; Gu S; Liu Z; Wang CZ; Li X; |
| Address: | "Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China. liuzewen@njau.edu.cn. State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, the Chinese Academy of Sciences, Beijing, 100101, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. lxc@email.arizona.edu. Department of Entomology and BIO5 Institute, The University of Arizona, Tucson, 85721, AZ, USA. lxc@email.arizona.edu" |
| DOI: | 10.1038/s41598-017-09185-2 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "How FACs-producing generalist and specialist herbivores regulate their FACs-hydrolyzing enzyme L-ACY-1 to balance FACs' beneficial vs. detrimental effects remains unknown. To address this question, we compared L-ACY-1 expression in Helicoverpa armigera and Helicoverpa assulta, a pair of closely related sibling species differing mainly in their host range, by the same sets of hostplants, protein to digestible carbohydrate (P:C) ratios, or allelochemical. L-ACY-1 expression remained low/unchanged in H. armigera, but was induced by hot pepper fruits and repressed by cotton bolls in H. assulta. The representative allelochemicals of the tested hostplants significantly (capsaicin) or insignificantly (gossypol and nicotine) induced L-ACY-1 expression in H. armigera, but insignificantly inhibited (capsaicin and gossypol) or induced (nicotine) it in H. assulta. L-ACY-1 expression remained low/unaltered on balanced (P50:C50 and P53:C47) or protein-biased diets and induced on carbohydrate-biased diets in H. armigera, but was at the highest level on balanced diets and reduced on either protein- or carbohydrate-biased diets in H. assulta. Furthermore, L-ACY-1 expression was significantly higher in H. assulta than in H. armigera for most of feeding treatments. Such expressional divergences suggest that FACs are utilized mainly for removal of excessive nitrogen in generalists but for nitrogen assimilation in specialists" |
| Keywords: | "Amidohydrolases/chemistry/genetics/*metabolism Amino Acid Sequence Amino Acids Animals Base Sequence Carbohydrates/analysis DNA, Complementary/genetics Digestive System/metabolism Fatty Acids Larva/enzymology Moths/*enzymology/genetics Pheromones/pharmaco;" |
| Notes: | "MedlineCheng, Qian Gu, Shaohua Liu, Zewen Wang, Chen-Zhu Li, Xianchun eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2017/08/20 Sci Rep. 2017 Aug 18; 7(1):8721. doi: 10.1038/s41598-017-09185-2" |
