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Mol Biol Cell

Title:		A yeast t-SNARE involved in endocytosis
Author(s):		Seron K; Tieaho V; Prescianotto-Baschong C; Aust T; Blondel MO; Guillaud P; Devilliers G; Rossanese OW; Glick BS; Riezman H; Keranen S; Haguenauer-Tsapis R;
Address:		"Institut Jacques Monod, Centre National de la Recherche Scientifique-UMRC7592, Universite Paris 7-Denis Diderot, Paris Cedex 05, France"
Journal Title:		Mol Biol Cell
Year:		1998
Volume:		9
Issue:		10
Page Number:		2873 - 2889
DOI:		10.1091/mbc.9.10.2873
ISSN/ISBN:		1059-1524 (Print) 1059-1524 (Linking)
Abstract:		"The ORF YOL018c (TLG2) of Saccharomyces cerevisiae encodes a protein that belongs to the syntaxin protein family. The proteins of this family, t-SNAREs, are present on target organelles and are thought to participate in the specific interaction between vesicles and acceptor membranes in intracellular membrane trafficking. TLG2 is not an essential gene, and its deletion does not cause defects in the secretory pathway. However, its deletion in cells lacking the vacuolar ATPase subunit Vma2p leads to loss of viability, suggesting that Tlg2p is involved in endocytosis. In tlg2Delta cells, internalization was normal for two endocytic markers, the pheromone alpha-factor and the plasma membrane uracil permease. In contrast, degradation of alpha-factor and uracil permease was delayed in tlg2Delta cells. Internalization of positively charged Nanogold shows that the endocytic pathway is perturbed in the mutant, which accumulates Nanogold in primary endocytic vesicles and shows a greatly reduced complement of early endosomes. These results strongly suggest that Tlg2p is a t-SNARE involved in early endosome biogenesis"
Keywords:		"Amino Acid Sequence Chromosomes, Fungal Cloning, Molecular Endocytosis/*physiology Fungal Proteins/biosynthesis/genetics Gene Deletion *Guanine Nucleotide Exchange Factors Kinetics Mating Factor Membrane Proteins/*chemistry/genetics/*metabolism Membrane T;"
Notes:		"MedlineSeron, K Tieaho, V Prescianotto-Baschong, C Aust, T Blondel, M O Guillaud, P Devilliers, G Rossanese, O W Glick, B S Riezman, H Keranen, S Haguenauer-Tsapis, R eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 1998/10/08 Mol Biol Cell. 1998 Oct; 9(10):2873-89. doi: 10.1091/mbc.9.10.2873"