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Dev Genet

Title:		Pheromone 4 gene of Euplotes octocarinatus
Author(s):		Meyer F; Schmidt HJ; Heckmann K;
Address:		"Institut fur Allgemeine Zoologie und Genetik, Universitat Munster, Federal Republic of Germany"
Journal Title:		Dev Genet
Year:		1992
Volume:		13
Issue:		1
Page Number:		16 - 25
DOI:		10.1002/dvg.1020130104
ISSN/ISBN:		0192-253X (Print) 0192-253X (Linking)
Abstract:		"We have cloned and sequenced a 1.7 kb macronuclear chromosome encoding the pheromone 4 gene of Euplotes octocarinatus. The sequence of the secreted pheromone is preceded by a 42 amino acid leader peptide, which ends with a lysine residue. The sequence coding for the leader peptide contains information for a putative signal peptide and is interrupted by a 772 bp intron as shown by comparison with a cDNA clone. A 64 bp intron and a 145 bp intron interrupt the sequence coding for the secreted pheromone. The three introns contain typical 5' and 3' splice junctions and a putative branch point site. The small introns have a low GC content. The large intron has a GC content similar to that of the pheromone 4 gene exons. The amino acid sequence of pheromone 4, deduced from both the genomic DNA and the cDNA of pheromone 4, shows that the secreted pheromone consists of 85 amino acids. One of its amino acids is encoded by a UGA codon. Since it has been shown for pheromone 3 of E. octocarinatus that UGA is translated as cysteine, it is assumed that the UGA codon encodes cysteine in pheromone 4 as well. The 164 bp noncoding region upstream of the leader peptide is AT-rich and contains an inverted repeat capable of forming a stem-loop structure with a stem of 11 bp. The 151 bp noncoding region at the 3' end of the chromosome contains a putative polyadenylation sequence and an inverted repeat. The macronuclear molecule is flanked by telomeres and carries the pentanucleotide motif TTGAA, located at a distance of 17 nucleotides from the telomeres. This motif has been suggested to be involved in the formation of macronuclear chromosomes"
Keywords:		"Amino Acid Sequence Animals Base Sequence Chromosome Mapping Cloning, Molecular DNA, Protozoan/isolation & purification Deoxyribonucleotides Euplotes/*genetics Molecular Sequence Data Pheromones/*genetics Protozoan Proteins/*genetics;"
Notes:		"MedlineMeyer, F Schmidt, H J Heckmann, K eng Research Support, Non-U.S. Gov't 1992/01/01 Dev Genet. 1992; 13(1):16-25. doi: 10.1002/dvg.1020130104"