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Proc Natl Acad Sci U S A

Title:		Antagonistic self-sensing and mate-sensing signaling controls antibiotic-resistance transfer
Author(s):		Chatterjee A; Cook LC; Shu CC; Chen Y; Manias DA; Ramkrishna D; Dunny GM; Hu WS;
Address:		"Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN 55455, USA"
Journal Title:		Proc Natl Acad Sci U S A
Year:		2013
Volume:		20130408
Issue:		17
Page Number:		7086 - 7090
DOI:		10.1073/pnas.1212256110
ISSN/ISBN:		1091-6490 (Electronic) 0027-8424 (Print) 0027-8424 (Linking)
Abstract:		"Conjugation is one of the most common ways bacteria acquire antibiotic resistance, contributing to the emergence of multidrug-resistant 'superbugs.' Bacteria of the genus Enterococcus faecalis are highly antibiotic-resistant nosocomial pathogens that use the mechanism of conjugation to spread antibiotic resistance between resistance-bearing donor cells and resistance-deficient recipient cells. Here, we report a unique quorum sensing-based communication system that uses two antagonistic signaling molecules to regulate conjugative transfer of tetracycline-resistance plasmid pCF10 in E. faecalis. A 'mate-sensing' peptide sex pheromone produced by recipient cells is detected by donor cells to induce conjugative genetic transfer. Using mathematical modeling and experimentation, we show that a second antagonistic 'self-sensing' signaling peptide, previously known to suppress self-induction of donor cells, also serves as a classic quorum-sensing signal for donors that functions to reduce antibiotic-resistance transfer at high donor density. This unique form of quorum sensing may provide a means of limiting the spread of the plasmid and present opportunities to control antibiotic-resistance transfer through manipulation of intercellular signaling, with implications in the clinical setting"
Keywords:		"Conjugation, Genetic/*physiology Drug Resistance, Bacterial/*genetics/physiology Enterococcus faecalis/*genetics/physiology *Models, Biological Plasmids/genetics Protein Sorting Signals/*genetics Quorum Sensing/*physiology Real-Time Polymerase Chain React;"
Notes:		"MedlineChatterjee, Anushree Cook, Laura C C Shu, Che-Chi Chen, Yuqing Manias, Dawn A Ramkrishna, Doraiswami Dunny, Gary M Hu, Wei-Shou eng T32 GM008347/GM/NIGMS NIH HHS/ R01 GM081388/GM/NIGMS NIH HHS/ GM49530/GM/NIGMS NIH HHS/ R01 GM049530/GM/NIGMS NIH HHS/ GM081888/GM/NIGMS NIH HHS/ T32GM008347/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't 2013/04/10 Proc Natl Acad Sci U S A. 2013 Apr 23; 110(17):7086-90. doi: 10.1073/pnas.1212256110. Epub 2013 Apr 8"