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Insect Biochem Mol Biol

Title:		RNA interference of pheromone biosynthesis-activating neuropeptide receptor suppresses mating behavior by inhibiting sex pheromone production in Plutella xylostella (L.)
Author(s):		Lee DW; Shrestha S; Kim AY; Park SJ; Yang CY; Kim Y; Koh YH;
Address:		"Ilsong Institute of Life Science, Hallym University, Anyang 431-060, Korea"
Journal Title:		Insect Biochem Mol Biol
Year:		2011
Volume:		20110108
Issue:		4
Page Number:		236 - 243
DOI:		10.1016/j.ibmb.2011.01.001
ISSN/ISBN:		1879-0240 (Electronic) 0965-1748 (Linking)
Abstract:		"Sex pheromone production is regulated by pheromone biosynthesis-activating neuropeptide (PBAN) in many lepidopteran species. We cloned a PBAN receptor (Plx-PBANr) gene from the female pheromone gland of the diamondback moth, Plutella xylostella (L.). Plx-PBANr encodes 338 amino acids and has conserved structural motifs implicating in promoting G protein coupling and tyrosine-based sorting signaling along with seven transmembrane domains, indicating a typical G protein-coupled receptor. The expression of Plx-PBANr was found only in the pheromone gland of female adults among examined tissues and developmental stages. Heterologous expression in human uterus cervical cancer cells revealed that Plx-PBANr induced significant calcium elevation when challenged with Plx-PBAN. Female P. xylostella injected with double-stranded RNA specific to Plx-PBANr showed suppression of the receptor gene expression and exhibited significant reduction in pheromone biosynthesis, which resulted in loss of male attractiveness. Taken together, the identified PBAN receptor is functional in PBAN signaling via calcium secondary messenger, which leads to activation of pheromone biosynthesis and male attraction"
Keywords:		"Amino Acid Sequence Animals Cell Line, Tumor Cloning, Molecular *Down-Regulation Female Humans Insect Proteins/*genetics/*metabolism Male Molecular Sequence Data Moths/classification/genetics/growth & development/*physiology Neuropeptides/metabolism Phylo;"
Notes:		"MedlineLee, Dae-Weon Shrestha, Sony Kim, A Young Park, Seok Joo Yang, Chang Yeol Kim, Yonggyun Koh, Young Ho eng Research Support, Non-U.S. Gov't England 2011/01/12 Insect Biochem Mol Biol. 2011 Apr; 41(4):236-43. doi: 10.1016/j.ibmb.2011.01.001. Epub 2011 Jan 8"