| Title: | Physical mapping of the conjugative bacteriocin plasmid pPD1 of Enterococcus faecalis and identification of the determinant related to the pheromone response |
| Author(s): | Fujimoto S; Tomita H; Wakamatsu E; Tanimoto K; Ike Y; |
| Address: | "Department of Microbiology, Gunma University School of Medicine, Japan" |
| DOI: | 10.1128/jb.177.19.5574-5581.1995 |
| ISSN/ISBN: | 0021-9193 (Print) 1098-5530 (Electronic) 0021-9193 (Linking) |
| Abstract: | "The pheromone-responding conjugative bacteriocin plasmid pPD1 (59 kb) of Enterococcus faecalis was mapped physically by using a relational clone approach, and transposon analysis with Tn917 (Emr) or Tn916 (Tcr) facilitated the location of the bacteriocin-related genes in a segment of about 6.7 kb. Tn917 insertions within a 3-kb region resulted in constitutive clumping. The nucleotide sequence of the region that included the insertions giving rise to constitutive clumping was determined. The region of pPD1 spanned about 8 kb and was found to contain a number of open reading frames, some of which were named on the basis of homologies with two other pheromone-responding plasmids, pAD1 and pCF10. The genes were arranged in the sequence repB-repA-traC-traB-traA-ipd-traE-traF- orfY-sea-1 with all but repB and traA oriented in the same (left-to-right) direction. traC and traB corresponded, respectively, to traC and traB of pAD1 and to prgY and prgZ of pCF10" |
| Keywords: | "Amino Acid Sequence Bacteriocins/biosynthesis/*genetics Base Sequence Cloning, Molecular Conjugation, Genetic/genetics Enterococcus faecalis/*genetics/metabolism Genes, Bacterial/genetics Genes, Regulator/genetics Molecular Sequence Data Mutagenesis, Inse;" |
| Notes: | "MedlineFujimoto, S Tomita, H Wakamatsu, E Tanimoto, K Ike, Y eng Research Support, Non-U.S. Gov't 1995/10/01 J Bacteriol. 1995 Oct; 177(19):5574-81. doi: 10.1128/jb.177.19.5574-5581.1995" |
