| Title: | "PBS2, a yeast gene encoding a putative protein kinase, interacts with the RAS2 pathway and affects osmotic sensitivity of Saccharomyces cerevisiae" |
| Address: | "Department of Medicine, Indiana University School of Medicine, Indianapolis 46202" |
| DOI: | 10.1099/00221287-138-11-2425 |
| ISSN/ISBN: | 0022-1287 (Print) 0022-1287 (Linking) |
| Abstract: | "The yeast gene PBS2 encodes a presumed protein kinase. The gene is essential for manifestation of resistance to the antibiotic polymyxin B. Deletion of PBS2 enables a ras2-530 null mutant to grow on nonfermentable carbon sources; overexpression of PBS2+ enhances viability of a RAS2Val19 mutant. Overexpression of PBS2+ also diminishes cellular response to mating pheromone MF alpha. These results suggest that the PBS2 and RAS2 genes affect a common pathway that may communicate with the pheromone response pathway. In addition, disruption of PBS2 renders cells sensitive to high osmolarity: exposure to 0.9 M-NaCl causes growth arrest, appearance of bizarre morphological forms, and eventual death. A mutation suppressing pbs2 deletion has been found. That mutation restores full polymyxin B resistance but only partially corrects the osmotic sensitivity defect. These observations indicate that PBS2 is involved in diverse physiological pathways in yeast" |
| Keywords: | "Carbon/metabolism Drug Resistance, Microbial Gene Deletion Genes, Fungal/*genetics Osmotic Fragility/*genetics Pheromones/pharmacology Polymyxin B/pharmacology Protein Kinases/*genetics Saccharomyces cerevisiae/cytology/drug effects/enzymology/*genetics *;" |
| Notes: | "MedlineBoguslawski, G eng England 1992/11/01 J Gen Microbiol. 1992 Nov; 138(11):2425-32. doi: 10.1099/00221287-138-11-2425" |
