| Title: | Use of TD-GC-TOF-MS to assess volatile composition during post-harvest storage in seven accessions of rocket salad (Eruca sativa) |
| Author(s): | Bell L; Spadafora ND; Muller CT; Wagstaff C; Rogers HJ; |
| Address: | "Department of Food and Nutritional Sciences, University of Reading, Whiteknights, PO Box 226, Reading, Berkshire RG6 6AP, UK. Electronic address: luke.bell@pgr.reading.ac.uk. Cardiff School of Biosciences, Cardiff University, Main Building, PO Box 915, Cardiff, CF10 3TL, UK. Department of Food and Nutritional Sciences, University of Reading, Whiteknights, PO Box 226, Reading, Berkshire RG6 6AP, UK; Centre for Food Security, University of Reading, Whiteknights, Reading, Berkshire RG6 6AP, UK" |
| DOI: | 10.1016/j.foodchem.2015.08.043 |
| ISSN/ISBN: | 1873-7072 (Electronic) 0308-8146 (Print) 0308-8146 (Linking) |
| Abstract: | An important step in breeding for nutritionally enhanced varieties is determining the effects of the post-harvest supply chain on phytochemicals and the changes in VOCs produced over time. TD-GC-TOF-MS was used and a technique for the extraction of VOCs from the headspace using portable tubes is described. Forty-two compounds were detected; 39 were identified by comparison to NIST libraries. Thirty-five compounds had not been previously reported in Eruca sativa. Seven accessions were assessed for changes in headspace VOCs over 7days. Relative amounts of VOCs across 3 time points were significantly different - isothiocyanate-containing molecules being abundant on 'Day 0'. Each accession showed differences in proportions/types of volatiles produced on each day. PCA revealed a separation of VOC profiles according to the day of sampling. Changes in VOC profiles over time could provide a tool for assessment of shelf life |
| Keywords: | "Brassicaceae/*chemistry *Gas Chromatography-Mass Spectrometry (E)-2-pentenal (PubChem CID: 5364752) (E)-4-oxohex-2-enal (PubChem CID: 6365145) (E, E)-2, 4-hexadienal (PubChem CID: 637564) 1-Isothiocyanato-3-methylbutane (PubChem CID: 79086) 1-Penten-3-ol (P;" |
| Notes: | "MedlineBell, Luke Spadafora, Natasha D Muller, Carsten T Wagstaff, Carol Rogers, Hilary J eng BB/J012629/1/Biotechnology and Biological Sciences Research Council/United Kingdom Research Support, Non-U.S. Gov't England 2015/10/17 Food Chem. 2016 Mar 1; 194:626-36. doi: 10.1016/j.foodchem.2015.08.043. Epub 2015 Aug 14" |
