| Title: | "Geosmin production and polyphasic characterization of Oscillatoria limosa Agardh ex Gomont isolated from the open canal of a large drinking water system in Tianjin City, China" |
| Author(s): | Cai F; Yu G; Zhang K; Chen Y; Li Q; Yang Y; Xie J; Wang Y; Li R; |
| Address: | "Key Laboratory of Algal Biology, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, People's Republic of China; University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China. Key Laboratory of Algal Biology, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, People's Republic of China. Tianjin Hydraulic Research Institute, Tianjin 300061, People's Republic of China. College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi, People's Republic of China. Key Laboratory of Algal Biology, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, People's Republic of China. Electronic address: reli@ihb.ac.cn" |
| DOI: | 10.1016/j.hal.2017.09.006 |
| ISSN/ISBN: | 1878-1470 (Electronic) 1568-9883 (Linking) |
| Abstract: | "Taste and odor (T & O) episodes always cause strong effects on drinking water supply system. Luanhe River diversion into Tianjin City in China is an important drinking water resource. Massive growth of a benthic filamentous cyanobacterium with geosmin production in the open canal caused a strong earthy odor episode in Tianjin. On the basis of the morphological and molecular identification of this cyanobacterium as Oscillatoria limosa Agardh ex Gomont, the genetic basis for geosmin biosynthesis and factors influencing growth and geosmin production of O. limosa CHAB 7000 were studied in this work. A 2268-bp open reading frame, encoding 755 amino acids, was amplified and characterized as the geosmin synthase gene (geo), followed by a cyclic nucleotide-binding protein gene (cnb). Phylogenetic analysis implied that the evolution of the geosmin genes in O. limosa CHAB 7000 might involve a horizontal gene transfer event. Examination on the growth and geosmin production of O. limosa CHAB 7000 at different light intensities showed that the maximum geosmin production was observed at 10mumol photons m(-2)s(-1), while the optimum growth was at 60mumol photons m(-2)s(-1). Under three temperature conditions (15 degrees C, 25 degrees C, and 35 degrees C), the maximum growth and geosmin production were observed at 25 degrees C. Most amounts of geosmin were retained in cells during the growth phase, but high temperature and low light intensity increased the release of geosmin into the medium, implying that O. limosa CHAB 7000 had a high potential harm for the release of geosmin from its cells at these adverse conditions" |
| Keywords: | "Base Sequence China Cities DNA, Ribosomal/genetics Drinking Water/*microbiology Geography Light Naphthols/*metabolism Oscillatoria/chemistry/genetics/growth & development/*isolation & purification Phylogeny Rivers/chemistry Temperature Volatile Organic Co;" |
| Notes: | "MedlineCai, Fangfang Yu, Gongliang Zhang, Kai Chen, Youxin Li, Qiang Yang, Yiming Xie, Jinlin Wang, Yilang Li, Renhui eng Research Support, Non-U.S. Gov't Netherlands 2017/11/11 Harmful Algae. 2017 Nov; 69:28-37. doi: 10.1016/j.hal.2017.09.006. Epub 2017 Oct 12" |
