| Title: | Ceramide signals for initiation of yeast mating-specific cell cycle arrest |
| Author(s): | Villasmil ML; Francisco J; Gallo-Ebert C; Donigan M; Liu HY; Brower M; Nickels JT; |
| Address: | "a The Institute of Metabolic Disorders, Genesis Biotechnology Group , Hamilton , NJ , USA. b Cato Research Ltd. , Durham , NC , USA. c Synthes, Inc , Paoli , PA , USA" |
| DOI: | 10.1080/15384101.2015.1127475 |
| ISSN/ISBN: | 1551-4005 (Electronic) 1538-4101 (Print) 1551-4005 (Linking) |
| Abstract: | "Sphingolipids are major constituents of membranes. A number of S. cerevisiae sphingolipid intermediates such as long chains sphingoid bases (LCBs) and ceramides act as signaling molecules regulating cell cycle progression, adaptability to heat stress, and survival in response to starvation. Here we show that S. cerevisiae haploid cells must synthesize ceramide in order to induce mating specific cell cycle arrest. Cells devoid of sphingolipid biosynthesis or defective in ceramide synthesis are sterile and harbor defects in pheromone-induced MAP kinase-dependent transcription. Analyses of G1/S cyclin levels indicate that mutant cells cannot reduce Cln1/2 levels in response to pheromone. FACS analysis indicates a lack of ability to arrest. The addition of LCBs to sphingolipid deficient cells restores MAP kinase-dependent transcription, reduces cyclin levels, and allows for mating, as does the addition of a cell permeable ceramide to cells blocked at ceramide synthesis. Pharmacological studies using the inositolphosphorylceramide synthase inhibitor aureobasidin A indicate that the ability to synthesize and accumulate ceramide alone is sufficient for cell cycle arrest and mating. Studies indicate that ceramide also has a role in PI(4,5)P2 polarization during mating, an event necessary for initiating cell cycle arrest and mating itself. Moreover, our studies suggest a third role for ceramide in localizing the mating-specific Ste5 scaffold to the plasma membrane. Thus, ceramide plays a role 1) in pheromone-induced cell cycle arrest, 2) in activation of MAP kinase-dependent transcription, and 3) in PtdIns(4,5)P2 polarization. All three events are required for differentiation during yeast mating" |
| Keywords: | "Alkaline Ceramidase Amidohydrolases/metabolism Cell Cycle Checkpoints/drug effects Cell Membrane/metabolism Ceramides/*metabolism Cyclins/metabolism Immunoblotting Microscopy, Fluorescence Mitogen-Activated Protein Kinases/metabolism Oxidoreductases/genet;" |
| Notes: | "MedlineVillasmil, Michelle L Francisco, Jamie Gallo-Ebert, Christina Donigan, Melissa Liu, Hsing-Yin Brower, Melody Nickels, Joseph T Jr eng Research Support, Non-U.S. Gov't 2016/01/05 Cell Cycle. 2016; 15(3):441-54. doi: 10.1080/15384101.2015.1127475. Epub 2016 Jan 4" |
