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J Bacteriol

Title:		Characterization of the traC determinant of the Enterococcus faecalis hemolysin-bacteriocin plasmid pAD1: binding of sex pheromone
Author(s):		Tanimoto K; An FY; Clewell DB;
Address:		"Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor 48109"
Journal Title:		J Bacteriol
Year:		1993
Volume:		175
Issue:		16
Page Number:		5260 - 5264
DOI:		10.1128/jb.175.16.5260-5264.1993
ISSN/ISBN:		0021-9193 (Print) 1098-5530 (Electronic) 0021-9193 (Linking)
Abstract:		"pAD1, a conjugative, 60-kb, hemolysin-bacteriocin plasmid in Enterococcus faecalis, encodes a mating response to a small peptide sex pheromone, cAD1, secreted by potential recipient bacteria. A gene, traC, encoding a 60.7-kDa protein with a typical amino terminal signal peptide, was identified within a region that appears to encode a product that binds to exogenous pheromone. A cloned segment of DNA containing traC resulted in specific binding of cells to synthetic cAD1. The putative traC product has strong similarity to a product of the E. faecalis plasmid pCF10 as well as oligopeptide binding proteins of Escherichia coli, Salmonella typhimurium, and Bacillus subtilis"
Keywords:		Amino Acid Sequence Bacterial Proteins/*genetics/metabolism Bacteriocins/genetics Base Sequence Enterococcus faecalis/*genetics/metabolism *Fimbriae Proteins Hemolysin Factors/genetics Molecular Sequence Data Oligopeptides/*metabolism Pheromones/*metaboli;
Notes:		"MedlineTanimoto, K An, F Y Clewell, D B eng GM33956/GM/NIGMS NIH HHS/ Comparative Study Research Support, U.S. Gov't, P.H.S. 1993/08/01 J Bacteriol. 1993 Aug; 175(16):5260-4. doi: 10.1128/jb.175.16.5260-5264.1993"