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J Agric Food Chem

Title:		Odorant-Binding Protein HvarOBP5 in Ladybird Hippodamia variegata Regulates the Perception of Semiochemicals from Preys and Habitat Plants
Author(s):		Tang H; Xie J; Liu J; Khashaveh A; Liu X; Yi C; Zhao D; He L; Sun Y; Zhang Y;
Address:		"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P. R. China. Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, P. R. China. College of Life Sciences, Anhui Normal University, Wuhu 241000, P. R. China. College of Animal Science, Shanxi Agricultral University, Jinzhong 030801, P. R. China. College of Plant Protection, Hebei Agricultural University, Baoding 017000, P. R. China"
Journal Title:		J Agric Food Chem
Year:		2023
Volume:		20230104
Issue:		2
Page Number:		1067 - 1076
DOI:		10.1021/acs.jafc.2c07355
ISSN/ISBN:		1520-5118 (Electronic) 0021-8561 (Linking)
Abstract:		"A total of 12 OBPs were identified in the antennae of ladybird Hippodamia variegata. HvarOBP1/2/5/6/10/11 were highly expressed in adults, whereas HvarOBP3/4/7/8/9/12 had higher expression levels in larvae. In adults, HvarOBP1/2/4/5/6/7/8/11/12 were highly expressed in antennae. Among these, recombinant HvarOBP5 strong bound with (E)-beta-farnesene (EbetaF), (R)-(+)-limonene, (E,E)-4,8,12-trimethyltrideca-l,3,7,11-tetraene (TMTT), (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT), hexyl hexanoate, and geranyl acetate. Molecular docking indicated that Leu42, Lys43, and His64 were the key binding sites of HvarOBP5. All six ligands evoked electroantennography (EAG) responses in ladybirds. Moreover, (R)-(+)-limonene and hexyl hexanoate were attractive to both sexes. After RNA interference for 72 h, the EAGs of dsRNA-injected ladybirds to DMNT and hexyl hexanoate were significantly decreased by 73.8 and 78.6%, respectively. Both dsRNA-injected males and females showed significantly lower behavioral preferences for DMNT and hexyl hexanoate. These findings suggest that HvarOBP5 in H. variegata plays an important role in the perception of semiochemical cues from preys and habitat plants"
Keywords:		"Female Male Animals *Pheromones/metabolism Caproates Limonene Molecular Docking Simulation *Receptors, Odorant/metabolism Perception Insect Proteins/genetics Arthropod Antennae/metabolism Hippodamia variegata RNA interference binding profiles electrophysi;"
Notes:		"MedlineTang, Haoyu Xie, Jiaoxin Liu, Jingtao Khashaveh, Adel Liu, Xiaoxu Yi, Chaoqun Zhao, Danyang He, Lin Sun, Yang Zhang, Yongjun eng 2023/01/05 J Agric Food Chem. 2023 Jan 18; 71(2):1067-1076. doi: 10.1021/acs.jafc.2c07355. Epub 2023 Jan 4"