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Sensors (Basel)

Title:		Dielectric Spectroscopy Based Detection of Specific and Nonspecific Cellular Mechanisms
Author(s):		Stoneman MR; Raicu V;
Address:		"Department of Physics, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USA. Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USA"
Journal Title:		Sensors (Basel)
Year:		2021
Volume:		20210503
Issue:		9
Page Number:		-
DOI:		10.3390/s21093177
ISSN/ISBN:		1424-8220 (Electronic) 1424-8220 (Linking)
Abstract:		"Using radiofrequency dielectric spectroscopy, we have investigated the impact of the interaction between a G protein-coupled receptor (GPCR), the sterile2 alpha-factor receptor protein (Ste2), and its cognate agonist ligand, the alpha-factor pheromone, on the dielectric properties of the plasma membrane in living yeast cells (Saccharomyces cerevisiae). The dielectric properties of a cell suspension containing a saturating concentration of alpha-factor were measured over the frequency range 40Hz-110 MHz and compared to the behavior of a similarly prepared suspension of cells in the absence of alpha-factor. A spherical three-shell model was used to determine the electrical phase parameters for the yeast cells in both types of suspensions. The relative permittivity of the plasma membrane showed a significant increase after exposure to alpha-factor (by 0.06 +/- 0.05). The equivalent experiment performed on yeast cells lacking the ability to express Ste2 showed no change in plasma membrane permittivity. Interestingly, a large change also occurred to the electrical properties of the cellular interior after the addition of alpha-factor to the cell suspending medium, whether or not the cells were expressing Ste2. We present a number of different complementary experiments performed on the yeast to support these dielectric data and interpret the results in terms of specific cellular reactions to the presence of alpha-factor"
Keywords:		"Cell Membrane *Dielectric Spectroscopy *Mating Factor Receptors, Mating Factor Saccharomyces cerevisiae *Saccharomyces cerevisiae Proteins G protein-coupled receptor Gpcr dielectric relaxation dielectric spectroscopy label-free detection ligand binding;"
Notes:		"MedlineStoneman, Michael R Raicu, Valerica eng PHY-1126386/National Science Foundation/ Switzerland 2021/06/03 Sensors (Basel). 2021 May 3; 21(9):3177. doi: 10.3390/s21093177"