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Microbiology (Reading)

Title:		Effects of the type III secreted pseudomonal toxin ExoS in the yeast Saccharomyces cerevisiae
Author(s):		Stirling FR; Evans TJ;
Address:		"Division of Immunology, Infection and Inflammation, University of Glasgow, Western Infirmary, Glasgow G11 6NT, UK"
Journal Title:		Microbiology (Reading)
Year:		2006
Volume:		152
Issue:		Pt 8
Page Number:		2273 - 2285
DOI:		10.1099/mic.0.28831-0
ISSN/ISBN:		1350-0872 (Print) 1350-0872 (Linking)
Abstract:		"Pseudomonas aeruginosa secretes a number of toxins by a type III system, and these are important in virulence. One of them, ExoS, is a bifunctional toxin, with a GTPase-activating protein domain, as well as ADP ribosyltransferase (ADPRT) activity. These two domains have numerous potential cellular targets, but the overall mechanism of ExoS action remains unclear. The effects of ExoS in a simple eukaryotic system, the yeast Saccharomyces cerevisiae, using a tetracycline-regulated expression system were studied. This system allowed controlled expression of ExoS in yeast, which was not possible using a galactose-induced system. ExoS was found to be an extremely potent inhibitor of yeast growth, and to be largely dependent on the activity of its ADPRT domain. ExoS produced a dramatic alteration in actin distribution, with the appearance of large aggregates of cortical actin, and thickened disorganized cables, entirely dependent on the ADPRT domain. This phenotype is suggestive of actin stabilization, which was verified by showing that the cortical aggregates of actin induced by ExoS were resistant to treatment with latrunculin A, an agent that prevents actin polymerization. ExoS increased the numbers of mating projections produced following growth arrest with mating pheromone, and prevented subsequent DNA replication, an effect that is again dependent on the ADPRT domain. Following pheromone removal, ExoS produced altered development of the mating projections, which became elongated with a swollen bud-like tip. These results suggest alternative pathways for ExoS action in eukaryotic cells that may result from activation of small GTPases, and this yeast expression system is well suited to explore these pathways"
Keywords:		ADP Ribose Transferases/chemistry/*physiology Actins/analysis/metabolism Bacterial Toxins/chemistry Base Sequence Cytoskeleton/chemistry DNA/biosynthesis Molecular Sequence Data Saccharomyces cerevisiae/*metabolism Tetracycline/pharmacology cdc42 GTP-Bind;
Notes:		"MedlineStirling, Fiona R Evans, Tom J eng Wellcome Trust/United Kingdom Research Support, Non-U.S. Gov't England 2006/07/20 Microbiology (Reading). 2006 Aug; 152(Pt 8):2273-2285. doi: 10.1099/mic.0.28831-0"