| Title: | Bioelectronic Nose Using Odorant Binding Protein-Derived Peptide and Carbon Nanotube Field-Effect Transistor for the Assessment of Salmonella Contamination in Food |
| Author(s): | Son M; Kim D; Kang J; Lim JH; Lee SH; Ko HJ; Hong S; Park TH; |
| Address: | "Interdisciplinary Program for Bioengineering, Seoul National University , Seoul 151-742, Korea. Department of Biophysics and Chemical Biology, Seoul National University , Seoul 151-742, Korea. School of Chemical and Biological Engineering, Seoul National University , Seoul 151-742, Korea. Bio-MAX Institute, Seoul National University , Seoul 151-818, Korea. Department of Physics and Astronomy and Institute of Applied Physics, Seoul National University , Seoul 151-747, Korea. Advanced Institutes of Convergence Technology , Suwon, Gyeonggi-do 443-270, Korea" |
| DOI: | 10.1021/acs.analchem.6b03284 |
| ISSN/ISBN: | 1520-6882 (Electronic) 0003-2700 (Linking) |
| Abstract: | "Salmonella infection is the one of the major causes of food borne illnesses including fever, abdominal pain, diarrhea, and nausea. Thus, early detection of Salmonella contamination is important for our healthy life. Conventional detection methods for the food contamination have limitations in sensitivity and rapidity; thus, the early detection has been difficult. Herein, we developed a bioelectronic nose using a carbon nanotube (CNT) field-effect transistor (FET) functionalized with Drosophila odorant binding protein (OBP)-derived peptide for easy and rapid detection of Salmonella contamination in ham. 3-Methyl-1-butanol is known as a specific volatile organic compound, generated from the ham contaminated with Salmonella. We designed and synthesized the peptide based on the sequence of the Drosophila OBP, LUSH, which specifically binds to alcohols. The C-terminus of the synthetic peptide was modified with three phenylalanine residues and directly immobilized onto CNT channels using the pi-pi interaction. The p-type properties of FET were clearly maintained after the functionalization using the peptide. The biosensor detected 1 fM of 3-methyl-1-butanol with high selectivity and successfully assessed Salmonella contamination in ham. These results indicate that the bioelectronic nose can be used for the rapid detection of Salmonella contamination in food" |
| Keywords: | "*Electronic Nose Food Contamination/*analysis Nanotubes, Carbon/*chemistry Peptides/*chemistry Receptors, Odorant/*chemistry Salmonella/*isolation & purification *Transistors, Electronic;" |
| Notes: | "MedlineSon, Manki Kim, Daesan Kang, Jinkyung Lim, Jong Hyun Lee, Seung Hwan Ko, Hwi Jin Hong, Seunghun Park, Tai Hyun eng Letter Research Support, Non-U.S. Gov't 2016/12/10 Anal Chem. 2016 Dec 6; 88(23):11283-11287. doi: 10.1021/acs.analchem.6b03284. Epub 2016 Nov 15" |
