| Title: | The Nicotiana attenuata GLA1 lipase controls the accumulation of Phytophthora parasitica-induced oxylipins and defensive secondary metabolites |
| Author(s): | Schuck S; Kallenbach M; Baldwin IT; Bonaventure G; |
| Address: | "Department of Molecular Ecology, Max Planck Institute of Chemical Ecology, D-07745, Jena, Germany" |
| ISSN/ISBN: | 1365-3040 (Electronic) 0140-7791 (Print) 0140-7791 (Linking) |
| Abstract: | "Nicotiana attenuata plants silenced in the expression of GLYCEROLIPASE A1 (ir-gla1 plants) are compromised in the herbivore- and wound-induced accumulation of jasmonic acid (JA). However, these plants accumulate wild-type (WT) levels of JA and divinyl-ethers during Phytophthora parasitica infection. By profiling oxylipin-enriched fractions with targeted and untargeted liquid chromatography-tandem time-of-flight mass spectrometry approaches, we demonstrate that the accumulation of 9-hydroxy-10E,12Z-octadecadienoic acid (9-OH-18:2) and additional C18 and C19 oxylipins is reduced by ca. 20-fold in P. parasitica-infected ir-gla1 leaves compared with WT. This reduced accumulation of oxylipins was accompanied by a reduced accumulation of unsaturated free fatty acids and specific lysolipid species. Untargeted metabolic profiling of total leaf extracts showed that 87 metabolites accumulated differentially in leaves of P. parasitica-infected ir-gla1 plants with glycerolipids, hydroxylated-diterpene glycosides and phenylpropanoid derivatives accounting together for ca. 20% of these 87 metabolites. Thus, P. parasitica-induced oxylipins may participate in the regulation of metabolic changes during infection. Together, the results demonstrate that GLA1 plays a distinct role in the production of oxylipins during biotic stress responses, supplying substrates for 9-OH-18:2 and additional C18 and C19 oxylipin formation during P. parasitica infection, whereas supplying substrates for the biogenesis of JA during herbivory and mechanical wounding" |
| Keywords: | "Chromatography, Liquid Fatty Acids/metabolism Lipase/*metabolism Lipid Metabolism Metabolomics Oxylipins/*metabolism Phytophthora/growth & development/*physiology Plant Diseases/microbiology Plant Extracts Plant Leaves/metabolism/microbiology *Secondary M;" |
| Notes: | "MedlineSchuck, Stefan Kallenbach, Mario Baldwin, Ian T Bonaventure, Gustavo eng 293926/ERC_/European Research Council/International Research Support, Non-U.S. Gov't 2014/01/24 Plant Cell Environ. 2014 Jul; 37(7):1703-15. doi: 10.1111/pce.12281. Epub 2014 Feb 24" |
